'Blood-doping' effects on hematocrit regulation and oxygen consumption in late-stage chicken embryos (Gallus gallus)
'Blood-doping' effects on hematocrit regulation and oxygen consumption in late-stage chicken embryos (Gallus gallus)
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DOI：
10.1242/jeb.014068
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发表时间：
2008-03-15
影响因子：
2.8
通讯作者：
Burggren, Warren
中科院分区：
文献类型：
--
作者：
Khorrami, Sheva;Tazawa, Hiroshi;Burggren, Warren
红细胞压积（Hct）的调节程度和改变Hct对鸟类胚胎血氧运输的影响在很大程度上是未知的。因此，我们研究了急性血液去除或林格氏溶液注射如何改变第15天胚胎的Hct，以及如何改变。用富含红细胞的全血进行“血液掺杂”影响了15-17天鸡胚的O-2消耗。平均Hct（+/- s.e.m.）在第15、16和17天分别为26.7 ± 0.6%、28.0 ± 0.4%和30.7 ± 0.5%。抽血（19次，每次125 μ l，间隔30分钟）导致Hct逐渐下降，第15天下降至12%。红细胞压积的下降与抽血的程度成正比。在8小时内递增林格氏液注射，瞬时增加血容量至初始值的85%，未降低Hct，表明注射的林格氏液迅速离开循环血室。在第15-17天胚胎中，用富含红细胞的全血进行血液掺杂，人为地将Hct从27%提高到38%，但在随后的6小时内的任何时间点，常规O-2消耗量（0.35-0.39 ml O-2 min（-1）egg（-1））均未引起显著变化。我们的结论是，红细胞压积是不保护急性在第15天的鸡胚，没有证据表明红细胞隔离或释放。此外，在第15-17天，Hct增加10%不会增加胚胎的耗氧量，这表明血液携氧能力本身并不局限于耗氧量。
The extent to which hematocrit ( Hct) is regulated and the impact of altered Hct on blood oxygen transport in avian embryos are largely unknown. Consequently, we investigated how acute blood removal or Ringer solution injection modified Hct in day 15 embryos, and how. blood doping' with erythrocyte-enriched whole blood influenced O-2 consumption in day 15-17 chicken embryos. Mean Hct ( +/- s.e.m.) at day 15, 16 and 17 was 26.7 +/- 0.6%, 28.0 +/- 0.4% and 30.7 +/- 0.5%, respectively. Blood withdrawal ( 19 increments of 125 mu l each, separated by 30 min) caused a progressive fall in Hct to similar to 12% at day 15. Hct decline was strictly proportional to the extent of blood withdrawal. Incremental Ringer solution injection over an 8 h period, transiently increasing blood volume up to 85% over initial values, did not decrease Hct, indicating that injected Ringer solution rapidly left the circulating blood compartment. Blood doping with erythrocyte-enriched whole blood artificially elevated Hct from 27% to 38%, but caused no significant change in routine O-2 consumption ( 0.35-0.39 ml O-2 min(-1) egg(-1)) at any point over the subsequent 6 h period in day 15-17 embryos. We conclude that Hct is not protected acutely in day 15 chicken embryos, with no evidence of erythrocyte sequestration or release. Additionally, at day 15-17, Hct increases of similar to 10% do not enhance embryonic oxygen consumption, suggesting that blood oxygen carrying capacity per se is not limiting to oxygen consumption.