Characterization of the F-box Proteins FBXW2 and FBXL14 in the Initiation of Bone Regeneration in Transplants given to Nude Mice.
Characterization of the F-box Proteins FBXW2 and FBXL14 in the Initiation of Bone Regeneration in Transplants given to Nude Mice.
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DOI：
10.2174/1874120701812010075
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Akiyama M
中科院分区：
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作者：
Akiyama M
培养的牛骨膜源性细胞无需人工支架材料即可在组织培养皿上形成三维结构，在体内可诱导骨再生。培养的牛骨膜来源细胞移植到裸鼠体内后用于骨组织再生已有报道，但确切的F-box分子机制尚不清楚。本研究的目的是研究体外培养的牛骨膜来源细胞骨再生所需的特异性F-box蛋白。本研究将骨膜组织和培养的骨膜来源细胞体外培养5周，然后用绿色组织标记染料包埋于胶原凝胶中。利用电泳和免疫组织化学鉴定组织骨再生所需的特异性F-box蛋白。在F-box蛋白表达后，观察到牛骨膜来源的细胞很快形成骨。骨形成初期，F-box蛋白停止表达。FBXW2在骨膜中表达，但在培养的骨膜来源细胞中不表达。此外，FBXL14在骨形成过程中消失。骨再生需要祖细胞，如牛骨膜来源的细胞和F-box蛋白FBXW2和FBXL14的激活，随着时间的推移，这些蛋白的表达停止。需要进一步的科学和临床试验来研究F-box蛋白如何用于治疗骨质疏松症和骨坏死。
Cultured bovine-periosteum-derived cells can form three-dimensional structures on tissue culture dishes without artificial scaffolding material, can induce bone regeneration in vivo. The utility of cultured bovine-periosteum-derived cells for bone tissue regeneration after their transplantation into nude mice has been reported, the precise F-box molecular mechanism was unclear. The aim of this study was to investigate the specific F-box proteins required for bone regeneration by cultured bovine-periosteum-derived cells in vitro. In the present study, periosteum tissue and cultured periosteum-derived cells were cultured for 5 weeks in vitro and then embedded in collagen gel with a green tissue-marking dye. Electrophoresis and immunohistochemistry were used to identify the specific F-box proteins required for tissue bone regeneration. The bovine-periosteum-derived cells were observed to form bone shortly after the expression of F-box proteins. After the initial phase of bone formation, the expression of the F-box proteins ceased. FBXW2 was shown to be expressed in the periosteum, but not in cultured periosteum-derived cells. Furthermore, FBXL14 disappeared during bone formation. Bone regeneration requires progenitor cells, such as bovine-periosteum-derived cells and the activation of the F-box Proteins FBXW2 and FBXL14, over time the expression of these proteins ceases. Further scientific and clinical trials are needed to investigate how the F-box Proteins can be used therapeutically to treat osteoporosis and osteonecrosis.