Frequent hereditable shutdown of murine retrovirus gene expression in murine cell lines.
Frequent hereditable shutdown of murine retrovirus gene expression in murine cell lines.
复制标题
鼠细胞系中鼠逆转录病毒基因表达的频繁遗传性关闭。
DOI：
10.1128/mcb.4.5.908-914.1984
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发表时间：
1984
影响因子：
5.3
通讯作者：
Kabat,D
中科院分区：
文献类型：
--
作者：
Bestwick,RK;Machida,CA;Polonoff,E;Kabat,D
Friend spleen focus-forming virus（Friend spleen focus-forming virus）经常关闭其基因表达。10 - 3/代），但在大鼠细胞中的频率要低得多（低于10 - 6/代）。因此，nonexpresser变异体被分离在高频率从鼠细胞系的免疫选择针对病毒编码的细胞表面糖蛋白，也简单地通过亚克隆细胞已培养了许多代。对独立感染的细胞克隆的研究表明，关闭是细胞系的性质，而不是特定前病毒位点的性质。核酸印迹分析表明，关闭与转录减少相关。此外，初步证据表明，其他鼠逆转录病毒也经常关闭在小鼠，但不是在大鼠细胞和关闭复制能力的小鼠白血病病毒伴随着损失的干扰重叠感染可能是限速反应，使细胞获得多个前病毒在其染色体。体内的高频关闭将具有重要的病理后果。
Friend spleen focus-forming virus shuts down its gene expression frequently (ca. 10−3per generation) in acis-dominant hereditable fashion in various murine cells but much less frequently in rat cells (less than 10−6per generation). Thus, nonexpresser variants were isolated at high frequency from murine cell lines by immunoselection directed against virus-encoded cell surface glycoproteins and also simply by subcloning cells from lines which had been cultured for many generations. Studies of independently infected cell clones indicate that shutdown is a property of the cell line rather than of the specific proviral site. Nucleic acid blot analyses suggest that shutdown correlates with decreased transcription. Moreover, preliminary evidence indicates that other murine retroviruses also shut down frequently in murine but not in rat cells and that shutdown of replication-competent murine leukemia viruses with accompanying loss in interference to superinfection may be the rate-limiting reaction enabling cells to acquire multiple proviruses in their chromosomes. High-frequency shutdown in vivo would have important pathological consequences.