The behavior of neural stem cells on biodegradable synthetic polymers
The behavior of neural stem cells on biodegradable synthetic polymers
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DOI：
10.1163/156856207779116711
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发表时间：
2007-01-01
影响因子：
3.6
通讯作者：
Kim, Byung-Soo
中科院分区：
文献类型：
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作者：
Bhang, Suk Ho;Lim, Jung Su;Kim, Byung-Soo
聚合物支架作为神经干细胞移植基质的生物相容性尚未得到广泛的研究。在这项研究中，我们评估了各种生物可降解聚合物对神经干细胞的生物相容性。生物相容性试验通过将海马祖细胞（HiB5）培养在聚乳酸-共乙醇酸（PLGA）、聚l -乳酸-共epsilon-己内酯（PLCL）和聚l -乳酸（PLLA）薄膜上或在这些聚合物提取物的存在下进行。具体来说，在生物相容性试验中检测HiB5细胞的活力、线粒体代谢活性、增殖、凋亡和神经突生长。在这些聚合物中，PLGA在细胞活力、线粒体代谢活性和凋亡活性方面表现最好。与其他聚合物相比，PLLA在所有类别中都表现出最差的结果。PLGA对HiB5细胞的神经突生长也有良好的效果。本研究结果证明了PLGA作为神经干细胞移植神经再生支架具有良好的生物相容性。
The biocompatibility of polymer scaffolds as neural stem cell transplantation matrices has not yet been studied extensively. In this study, we evaluated the biocompatibility of various biodegradable polymers for neural stem cells. The biocompatibility tests were performed by culturing hippocampal progenitor cells (HiB5) on films of poly(lactic-co-glycolic acid) (PLGA), poly(L-lactide-co-epsilon-caprolactone) (PLCL) and poly(L-lactic acid) (PLLA) or in the presence of extracts from these polymers. Specifically, the viability, mitochondrial metabolic activity, proliferation, apoptosis and neurite out-growth of HiB5 cells were examined in biocompatibility tests. Among the tested polymers, PLGA performed best with respect to cell viability, mitochondrial metabolic activity and apoptotic activity. Compared to the other polymers, PLLA showed the worst results in all categories evaluated. PLGA also showed favorable results for neurite out-growth of HiB5 cells. The results of this study demonstrate the promising biocompatibility of PLGA as a scaffold for neural stem cell transplantation for nerve regeneration.