A null allele of the pol IV second subunit impacts stature and reproductive development in Oryza sativa
A null allele of the pol IV second subunit impacts stature and reproductive development in Oryza sativa
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DOI：
10.1111/tpj.15848
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发表时间：
2022-06-22
期刊：
影响因子：
7.2
通讯作者：
Mosher, Rebecca A.
中科院分区：
文献类型：
--
作者：
Chakraborty, Tania;Trujillo, Joshua T.;Mosher, Rebecca A.
所有真核生物都有三种DNA依赖的RNA聚合酶，Pol I-III，而陆生植物有另外两种聚合酶，Pol IV和Pol V。Pol IV通过Pol II亚基的复制衍生，产生24-nt短干扰RNA，与Pol V转录物相互作用，以靶DNA甲基化和沉默转座子的转录。草科的成员编码额外的重复的Pol IV和V亚基，提出了关于每个平行函数的问题。在这项研究中，我们确定了Pol IV第二亚基NRPD2的一个空等位基因，并证明NRPD2是与NRPD1一起在叶片中产生小RNA和CHH甲基化的唯一亚基。纯合子nrpd2突变体既没有配子体缺陷，也没有胚胎致死性，尽管成年植株矮小且不育。
All eukaryotes possess three DNA-dependent RNA polymerases, Pols I-III, while land plants possess two additional polymerases, Pol IV and Pol V. Derived through duplication of Pol II subunits, Pol IV produces 24-nt short interfering RNAs that interact with Pol V transcripts to target de novo DNA methylation and silence transcription of transposons. Members of the grass family encode additional duplicated subunits of Pol IV and V, raising questions regarding the function of each paralog. In this study, we identify a null allele of the putative Pol IV second subunit, NRPD2, and demonstrate that NRPD2 is the sole subunit functioning with NRPD1 in small RNA production and CHH methylation in leaves. Homozygous nrpd2 mutants have neither gametophytic defects nor embryo lethality, although adult plants are dwarf and sterile.