Inhibition of autoantibody binding to platelet glycoprotein IIb/IIIa by anti-idiotypic antibodies in intravenous gammaglobulin
Inhibition of autoantibody binding to platelet glycoprotein IIb/IIIa by anti-idiotypic antibodies in intravenous gammaglobulin
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静脉注射丙种球蛋白中抗独特型抗体抑制自身抗体与血小板糖蛋白 IIb/IIIa 的结合
DOI：
10.1182/blood.v74.7.2414.bloodjournal7472414
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发表时间：
1989
期刊：
影响因子：
20.3
通讯作者：
R. Mcmillan
中科院分区：
文献类型：
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作者：
P. Berchtold;G. Dale;P. Tani;R. Mcmillan
静脉注射免疫球蛋白（IVIgG）可引起大多数慢性免疫性血小板减少性紫癜（ITP）患者血小板计数的急性升高，但其作用机制尚不清楚。我们评估了三种不同的IVIgG制剂抑制自身抗体与血小板糖蛋白（GP）IIb/IIIa体外结合的能力。将已知含有抗GPIIb/IIIa抗体的ITP血浆与IVIgG或牛血清白蛋白（BSA）孵育过夜，然后测量自身抗体滴度。8例ITP患者的自身抗体结合被IVIgG抑制，与IVIgG浓度成比例。使用与体内预期治疗浓度相容的3.2%IVIgG，自身抗体结合的平均抑制范围为20.2%至41.3%。未检测到IVIgG对同种异体抗体与相同或不同分子结合的抑制（5例抗GPIIb/IIIa患者和2例抗HLA同种异体抗体患者）。IVIgG的F（ab '）2片段也抑制血浆自身抗体和通过从抗原亲和柱洗脱制备的纯化的抗GPIIb/IIIA自身抗体的结合。部分抗独特型抗体可被纯化的抗GPIIb/IIIa自身抗体吸附。这些结果表明，来自正常供体的IVIgG制剂含有针对位于GPIIb/IIIa自身抗体上的独特型的抗独特型抗体，但不具有针对相同或不同分子的血小板同种异体抗体的抗独特型。这些抗独特型抗体在IVIgG治疗反应中的重要性仍有待确定。
Intravenous immunoglobulin (IVIgG) causes an acute rise in the platelet count in the majority of patients with chronic immune thrombocytopenic purpura (ITP) but the mechanism(s) of action is still unknown. We evaluated the ability of three different IVIgG preparations to inhibit the in vitro binding of autoantibody to platelet glycoprotein (GP) IIb/IIIa. ITP plasma, known to contain anti-GPIIb/IIIa antibodies, was incubated overnight with either IVIgG or bovine serum albumin (BSA) followed by measurement of the autoantibody titer. Binding of autoantibody from eight ITP patients was inhibited by IVIgG in proportion to the IVIgG concentration. Using 3.2% IVIgG, compatible with therapeutic concentrations expected in vivo, mean inhibition of autoantibody binding ranged from 20.2% to 41.3%. No inhibition by IVIgG of alloantibody binding to the same or different molecules was detected (five patients with anti-GPIIb/IIIa and two with anti-HLA alloantibodies). F(ab')2 fragments of IVIgG also inhibited the binding of both plasma autoantibodies and purified anti-GPIIb/IIIA autoantibodies prepared by elution from antigen affinity columns. A portion of the anti-idiotypic antibodies could be adsorbed from IVIgG using insolubilized, purified anti-GPIIb/IIIa autoantibody. These results show that IVIgG preparations from normal donors contain anti-idiotypic antibodies directed against idiotypes located on GPIIb/IIIa autoantibodies but do not have anti-idiotypes to platelet alloantibodies against the same or different molecules. The importance of these anti-idiotypic antibodies in the therapeutic response to IVIgG remains to be established.
影响因子：
20.3
作者：
R. Mcmillan;P. Tani;F. Millard;P. Berchtold;Renshaw Lw;V. Woods
通讯作者：
R. Mcmillan;P. Tani;F. Millard;P. Berchtold;Renshaw Lw;V. Woods
独特型 B 细胞淋巴瘤的调节。
DOI：
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发表时间：
1988
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Ward,RE;McNamara-Ward,M;Webb,CF;Altman,D;Lim,PL;Tucker,PW;Kohler,H
通讯作者：
Kohler,H