METABOLISM IN VIVO OF 1,1,1-TRICHLORO-2,2-BIS(P-CHLOROPHENYL)ETHANE (DDT) 1,1-DICHLORO-2,2-BIS(P-CHLOROPHENYL)ETHANE (DDD) AND 1,1-DICHLORO-2,2-BIS(P-CHLOROPHENYL)ETHYLENE (DDE) IN CHICK BY EMBRYONIC INJECTION AND DIETARY INGESTION
METABOLISM IN VIVO OF 1,1,1-TRICHLORO-2,2-BIS(P-CHLOROPHENYL)ETHANE (DDT) 1,1-DICHLORO-2,2-BIS(P-CHLOROPHENYL)ETHANE (DDD) AND 1,1-DICHLORO-2,2-BIS(P-CHLOROPHENYL)ETHYLENE (DDE) IN CHICK BY EMBRYONIC INJECTION AND DIETARY INGESTION
复制标题
DOI：
10.1016/0006-2952(68)90189-5
复制
发表时间：
1968-01-01
影响因子：
5.8
通讯作者：
MENZEL, DB
中科院分区：
文献类型：
--
作者：
ABOUDONIA, MB;MENZEL, DB
用花生油分别注射1，1，1-三氯-2，2-双（对氯苯基）乙烷（p，p′-DDT）、1，1-二氯-2，2-双（对氯苯基）乙烷（p，p′-DDD）和1，1-二氯-2，2-双（对氯苯基）乙烯（DDE）于受精卵孵化前，研究其代谢及对胚胎和雏鸡的影响。在雏鸡或死亡胚胎中均未检测到致畸作用。观察到典型的DDT中毒神经症状。在其他试验中，将饲料中100 ppm的p，p′-DDT、p，p′-DDD和DDE喂给从未经处理的蛋中孵化出的雏鸡。采用电子捕获气液色谱法和薄层色谱法对注射和饲喂试验中的雏鸡组织进行了代谢物分析。红外光谱法与TLC结合使用，以确认某些极性代谢物的鉴别。未观察到两种处理之间代谢物模式的显著差异。从mp，p′-DDT中检测到以下代谢产物：2-邻氯苯基（对氯苯基）-1，1，1-三氯乙烷，（o，p′-DDT）; DDE;p，p′-DDD; 2-邻氯苯基（对氯苯基）-1，1-二氯乙烷，（o，p'-DDD）; 1-chloro-2，2-bis-（p-chlorophenyl）ethylene，（DDMU）; 1-chloro-2，2-bis对氯苯基乙烷对氯苯基乙烯（DDNU）; 2，2-双（对氯苯基）乙醇（DDOH）;双（对氯苯基）乙酸（DDA）;双（对氯苯基）甲烷（DDM）和4，4 '-二氯二苯甲酮（DBP）。从p，p '-DDD中检测到以下代谢物：o，p-DDD; DDMU; DDMS; DDNU; DDOH; DDA; DDM和DBP。DBP被检测为DDE的代谢产物。
1,1,1 -Trichloro-2,2-bis(p-chlorophenyl)ethane, (p,p′-DDT) ; 1,1 -dichloro-2,2-bis(p-chlorophenyl)ethane, (p,p′-DDD); and 1,1-dichloro-2,2-bis(p-chlorophenyl)-ethylene, (DDE) were separately injected in peanut oil into fertile eggs prior to incubation to study their metabolism and effect on the embryo and chicks. No teratogenic effect was detected either in chicks or in dead embryos. Typical DDT poisoning neurological symptoms were observed. In other experiments, 100 ppm ofp,p′-DDT,p,p′-DDD and DDE in the diet were fed to chicks hatched from untreated eggs. Tissues of chicks in both the injection and feeding experiments were analyzed for metabolites by electron capture gas-liquid and thin-layer chromatography (TLC). Infrared spectroscopy was used in connection with TLC for confirmation of the identity of some polar metabolites. No significant differences in the pattern of metabolites between the two treatments were observed. The following metabolites were detected fromp,p′-DDT: 2-(o-chlorophenyl)-2-(p-chlorophenyl)-1,1,1-trichloroethane, (o,p′-DDT); DDE;p,p′-DDD; 2-(o-chlorophenyl)-2-(p-chlorophenyl)-1,1-dichloro-ethane, (o,p′-DDD); 1-chloro-2,2-bis-(p-chlorophenyl)ethylene, (DDMU); 1-chloro-2,2-bis(p-crilorophenyl)ethane, (DDMS); unsym-bis(p-chlorophenyl)ethylene, (DDNU); 2,2-bis(p-chlorophenyl)-ethanol, (DDOH);bis(p-chlorophenyl)acetic acid, (DDA);bis(p-chloropheny)-methane, (DDM) and 4,4'-dichlorobenzophenone, (DBP). The following metabolites were detected fromp,p'-DDD:o,p-DDD; DDMU; DDMS; DDNU; DDOH; DDA; DDM and DBP. DBP was detected as a metabolite of DDE.