Cryopreservation of Papaya (Carica papaya L.) Seed: Overcoming Dormancy and Optimizing Seed Desiccation and Storage Conditions
Cryopreservation of Papaya (Carica papaya L.) Seed: Overcoming Dormancy and Optimizing Seed Desiccation and Storage Conditions
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DOI：
10.17660/actahortic.2009.839.28
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发表时间：
2009-07
期刊：
影响因子：
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通讯作者：
S. Ashmore;R. Drew;C. O'brien;A. Parisi
中科院分区：
文献类型：
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作者：
S. Ashmore;R. Drew;C. O'brien;A. Parisi
业界需要有效储存和发芽新鲜和干燥的番木瓜种子的协议，以改善种子库存的管理。研究了对澳大利亚具有重要意义的两种木瓜基因型：热带品种‘Solo’和昆士兰品种‘007’。新鲜种子的萌发率接近于零，但通过四氮唑染色发现种子的存活率很高：‘Solo’为93%，‘007’为94%。我们的目标是通过在赤霉素酸（长达60分钟）或硝酸钾（长达24小时）中预浸泡种子来克服休眠阻塞。最有效的处理是：2mmga3处理30min；和2或3h在0.25 M kno3。所有处理的发芽率均为61 ~ 65%。两个品种的种子都能耐受干燥至低含水量，但需要GA 3预处理以促进30-60%的萌发；kno3预处理对刺激干燥种子萌发的效果较差。如果使用GA 3预处理，干燥的种子（5%、15%和30% RH）在液氮（-196°C）中储存3个月后发芽（>70%）。在-20°C标准基因库储存温度下的恢复率非常低（1-36%）。这些结果为改进番木瓜产业的种子处理方法提供了有价值的信息。
Protocols for efficient storage and germination of fresh and desiccated seeds of Carica papaya are needed for improved management of seed stocks by the industry. Two genotypes of papaya of importance to Australia were investigated: the tropical cultivar 'Solo' and a Queensland line '007'. Germination of fresh seed was close to zero, but high proportions of the seeds were found to be viable by tetrazolium staining: 93% for 'Solo' and 94% for '007'. We aimed to overcome the dormancy blocking by pre-soaking seeds in gibberellic acid (for up to 60 min) or potassium nitrate (for up to 24h). The most effective treatments were: 2 mM GA 3 for 30 min; and 2 or 3h in 0.25 M KNO 3 . All treatments resulted in 61-65% germination. Seeds of both cultivars tolerated drying to low moisture contents, but required GA 3 pre-treatment to stimulate 30-60% germination; KN0 3 pre-treatment was much less effective in stimulating germination in desiccated seeds. Desiccated seeds (5, 15 and 30% RH) germinated (>70%) after storage in liquid nitrogen(-196°C) for 3 months if GA 3 pre-treatment was applied. Recovery from storage at the standard genebank storage temperature of -20°C was very low (1-36%). These results provide valuable information for improved seed handling practices in the papaya industry.