ULTRASTRUCTURE OF SUBSTANCE-P-IMMUNOREACTIVE AND CGRP-IMMUNOREACTIVE NERVE-FIBERS IN THE NASAL EPITHELIUM OF RODENTS
ULTRASTRUCTURE OF SUBSTANCE-P-IMMUNOREACTIVE AND CGRP-IMMUNOREACTIVE NERVE-FIBERS IN THE NASAL EPITHELIUM OF RODENTS
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DOI：
10.1002/cne.902940212
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发表时间：
1990-04-08
影响因子：
2.5
通讯作者：
SILVER, WL
中科院分区：
文献类型：
--
作者：
FINGER, TE;STJEOR, VL;SILVER, WL
在超微结构水平上检查了大鼠和小鼠的呼吸和嗅觉粘膜上皮内神经末梢的存在。免疫细胞化学研究，利用抗血清直接对P物质和降钙素基因相关肽（CGRP）显示许多上皮内肽免疫反应纤维附近的基底区域的上皮。偶尔观察到跨上皮纤维向外延伸，几乎到达上皮表面。然而，在任何情况下，跨上皮纤维都没有到达表面，而是在距离表面约1 μ m的紧密连接线处停止，没有观察到神经纤维和上皮细胞之间的专门接触。经上皮纤维为三叉神经对许多空气传播的化学刺激的敏感性提供了可能的解剖学基础。潜在的化学刺激必须穿过紧密连接屏障，这可以解释为什么脂溶性与三叉神经刺激的有效性有关。
The respiratory and olfactory mucosae of rats and mice were examined at ultrastructural levels for the presence of intraepithelial nerve endings. Immunocytochemical studies utilizing antisera directed against substance P and calcitonin gene-related peptide (CGRP) revealed numerous intraepithelial peptide-immunoreactive fibers near the basal region of the epithelium. Occasional transepithelial fibers were observed to extend outward to nearly reach the epithelial surface. In no cases, however, did the transepithelial fibers reach the surface, but instead, stopped at the line of tight junctions approximately 1 .mu.m from the surface, no specialized contacts between the nerve fibers and the epithelial cells were observed. The transepithelial fibers provide a possible anatomical substrate for the sensitivity of the trigeminal nerve to many air-borne chemical stimuli. That potential chemical stimuli must traverse the tight-junctional barrier may explain why lipid solubility is related to effectiveness for trigeminal stimuli.