Chitosan-dextran sulfate nanoparticles for delivery of an anti-angiogenesis peptide
Chitosan-dextran sulfate nanoparticles for delivery of an anti-angiogenesis peptide
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DOI：
10.1007/bf02442596
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发表时间：
2003-01-01
期刊：
影响因子：
--
通讯作者：
Parkin, JE
中科院分区：
文献类型：
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作者：
Chen, Y;Mohanraj, VJ;Parkin, JE
利用壳聚糖（CS）和硫酸葡聚糖（DS）两种电荷相反的聚合物，通过简单的凝聚过程，开发了一种新型的纳米粒给药系统。在所研究的条件下，两种聚合物的重量比被确定为控制所产生的纳米颗粒的粒径、表面电荷、包封效率和释放特性的决定因素。在最佳条件下产生平均直径为223 nm的颗粒，ζ电位约为-32.6 mV。CS：DS重量比为0.59：1时，抗血管生成肽的包封率最高可达75%。相同的纳米颗粒制剂也显示出在6天的时间内缓慢和持续的肽释放。相比之下，发现含有较低比例的CS：DS（0.5：1）的制剂具有降低的包封效率和更快速的肽释放特性。本研究的结果表明，CS-DS纳米粒的物理化学和释放特性可以通过改变两种离子聚合物的比例来调节。新型CS-DS纳米颗粒。通过凝聚法制备的多肽具有作为小肽载体的潜力。
A novel nanoparticle delivery system has been developed by employing the oppositely charged polymers chitosan (CS) and dextran sulfate (DS), and a simple coacervation process. Under the conditions investigated, the weight ratio of the two polymers is identified as a determining factor controlling particle size, surface charge, entrapment efficiency and release characteristics of the nanoparticles produced. Particles of 223 nm mean diameter were produced under optimal conditions with a zeta potential of approximately -32.6 mV. A maximum of 75% anti-angiogenesis peptide entrapment efficiency was achieved with a CS:DS weight ratio of 0.59:1. The same nanoparticle formulation also showed slow and sustained peptide release over a period of 6 days. In contrast, the formulation containing a lower ratio of CS:DS (0.5:1) was found to have reduced entrapment efficiency and more rapid peptide release characteristics. The results of this study suggest that physicochemical and release characteristics of the CS-DS nanoparticles can be modulated by changing ratios of two ionic polymers. The novel CS-DS nanoparticles. prepared by the coacervation process have potential as a carrier for small peptides.