Corrosion inhibition by aerobic biofilms on SAE 1018 steel
Corrosion inhibition by aerobic biofilms on SAE 1018 steel
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DOI：
10.1007/s002530050889
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发表时间：
1997-01-01
影响因子：
5
通讯作者：
Wood, TK
中科院分区：
文献类型：
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作者：
Jayaraman, A;Earthman, JC;Wood, TK
将碳钢 (SAE 1018) 样品暴露于含有需氧细菌 Pseudomonas fragi 或兼性厌氧菌 Escherichia coli DH5 alpha 的复杂液体介质中。与无菌对照相比，在这些产生保护性生物膜的细菌存在的情况下，质量损失始终低 2 至 10 倍。将温度从 23 摄氏度升高至 30 摄氏度，导致 P. fragi 的腐蚀抑制作用降低 2 至 5 倍，而同样的温度变化导致 E. coli DH5 α 的腐蚀抑制作用增加 2 倍。在不形成生物膜的浅青紫链霉菌 TK24 中观察到的腐蚀与在无菌介质中观察到的腐蚀相似。通过向已建立的生物膜中添加卡那霉素原位生成的死生物膜不能保护金属（腐蚀率与无菌对照中的腐蚀率相当），并且无细胞废 Luria-Bertani (LB) 培养基中的质量损失与无菌培养基中的质量损失相似。共焦激光扫描显微镜分析证实存在由嵌入稀疏糖萼基质中的活细胞和死细胞组成的生物膜。质量损失测量结果与暴露两周后金属表面的显微镜观察结果一致，表明发生了均匀腐蚀。生物膜还能够承受轻微的搅拌（60 rpm），前提是有足够的时间来发展。
Carbon steel (SAE 1018) samples were exposed to complex liquid media containing either the aerobic bacterium Pseudomonas fragi or the facultative anaerobe Escherichia coli DH5 alpha. Compared to sterile controls, mass loss was consistently 2- to 10-fold lower in the presence of these bacteria which produce a protective biofilm. Increasing the temperature from 23 degrees C to 30 degrees C resulted in a 2- to 5-fold decrease in corrosion inhibition with P. fragi whereas the same shift in temperature resulted in a 2-fold increase in corrosion inhibition with E. coli DH5 alpha. Corrosion observed with non-biofilm-forming Streptomyces lividans TK24 was similar to that observed in sterile media. A dead biofilm, generated in situ by adding kanamycin to an established biofilm, did not protect the metal (corrosion rates were comparable to those in the sterile control), and mass loss in cell-free, spent Luria-Bertani (LB) medium was similar to that in sterile medium. Confocal laser scanning microscopy analysis confirmed the presence of a biofilm consisting of live and dead cells embedded in a sparse glycocalyx matrix. Mass-loss measurements were consistent with microscopic observations of the metal surface after 2 weeks of exposure, indicating that uniform corrosion occurred. The biofilm was also able to withstand mild agitation (60 rpm), provided that sufficient time was given for its development.