Serological and molecular evidence of Coxiella burnetii in samples from humans and animals in China
Serological and molecular evidence of Coxiella burnetii in samples from humans and animals in China
复制标题
中国人类和动物样本中伯氏柯克斯体的血清学和分子证据。
DOI：
10.5604/12321966.1196859
复制
发表时间：
2016-01-01
影响因子：
1.7
通讯作者：
Wang, Chengming
中科院分区：
文献类型：
--
作者：
El-Mahallawy, Heba S.;Kelly, Patrick;Wang, Chengming
介绍
贝氏柯克斯体是Q热的病原体，Q热是一种世界性的人畜共患病。为了增加中国对该病的认识，C。在中国东部江苏省扬州市的五种动物和人类的方便样品中调查了伯奈蒂感染。
材料和方法
商业ELISA试剂盒用于检测I相和II C相的抗体。伯内特氏菌同时建立了以外膜蛋白com 1基因为靶点的FRET qPCR方法。来自动物和人类的血液样品以及牛奶样品中的贝氏体DNA。
结果
发现血清阳性牛（44/150; 29%）、山羊（33/150; 22%）、人（45/180; 25%）和猪（4/130; 3%），而犬（0/136; 0%）和猫（0/140; 0%）为血清阴性。人类血清阳性与年龄增长有关，但没有性别差异。从2份牛奶样品中扩增出DNA（2/150，1.3%），而血液样品中无阳性。获得的扩增子的序列与通用C. BurnetiiRSA 493菌株和中国其他菌株。
结论
结果表明，C.贝氏体在中国扬州是地方性的，因此人类和动物卫生工作者应该意识到Q热感染的可能性和暴发的发生。
INTRODUCTION
Coxiella burnetii is the agent of Q fever, a worldwide zoonosis. To add to the available knowledge of the disease in China, C. burnetti infections were investigated in convenience samples from five animal species and humans from Yangzhou, Jiangsu province, eastern China.
MATERIALS AND METHODS
Commercial ELISA kits were used to detect antibodies to phase I and II C. burnetii. A FRET-qPCR targeting the outer membrane protein com1 gene was also developed to detect C. burnetii DNA in blood samples from animals and humans, and bovine milk samples.
RESULTS
Seropositive cattle (44/150; 29%), goats (33/150; 22%), humans (45/180; 25%) and pigs (4/130; 3%) were found, while dogs (0/136; 0%) and cats (0/140; 0%) were seronegative. Seropositivity in humans was associated with increasing age, but there was no gender difference. DNA was amplified from two milk samples (2/150, 1.3%), while none of the blood samples were positive. The sequences of the obtained amplicons were identical to those of the com1 gene of the universal C. burnetii RSA 493 strain and other stains from China.
CONCLUSIONS
The findings indicaten that C. burnetii is endemic in Yangzhou, China, and therefore human and animal health workers should be aware of the possibility of infections and the occurrence of outbreaks of Q fever.