Insulin Binding and Effects of Insulin on Glucose Uptake and Metabolism in Cultured Rabbit Coronary Microvessel Endothelium
Insulin Binding and Effects of Insulin on Glucose Uptake and Metabolism in Cultured Rabbit Coronary Microvessel Endothelium
复制标题
胰岛素结合以及胰岛素对培养兔冠状动脉微血管内皮细胞葡萄糖摄取和代谢的影响
DOI：
10.3181/00379727-180-42137
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发表时间：
1985
影响因子：
--
通讯作者：
Theresa M. Burke
中科院分区：
文献类型：
--
作者：
M. Gerritsen;Theresa M. Burke
摘要从兔心肌组织中分离出微血管内皮细胞，采用标准的组织培养技术进行培养。胰岛素可显著促进[U-14C]葡萄糖向二氧化碳和血脂的转化。胰岛素促进2-脱氧葡萄糖的摄取，促进3-O-[~3H]甲基-D-葡萄糖的转运。还观察到[125I]胰岛素与RCME细胞的特异性结合，这种结合可被冷胰岛素取代。这些数据表明，胰岛素能够调节冠脉微血管内皮细胞的代谢活动。
Abstract Microvascular endothelial cells were isolated from rabbit cardiac tissue, and cultivated by standard tissue culture techniques. The conversion of [U-14C]glucose to CO2 and lipids was significantly enhanced by insulin treatment. Insulin stimulated uptake of 2-[3H]deoxyglucose and enhanced the transport of 3-O[3H]methyl-D-glucose. Specific binding of [125I]insulin to RCME cells, displaceable by cold insulin, was also observed. These data demonstrate that insulin is capable of regulating metabolic activities in coronary microvascular endothelium.
胰岛素与完整心脏的微血管内皮结合：动力学和形态分析。
DOI：
10.1152/ajpendo.1983.244.5.e447
发表时间：
1983
期刊：
The American journal of physiology
影响因子：
--
作者：
Bar,RS;DeRose,A;Sandra,A;Peacock,ML;Owen,WG
通讯作者：
Owen,WG