Beamline B21: high-throughput small-angle X-ray scattering at Diamond Light Source
Beamline B21: high-throughput small-angle X-ray scattering at Diamond Light Source
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DOI：
10.1107/s1600577520009960
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发表时间：
2020-09-01
影响因子：
2.5
通讯作者：
Rambo, Robert P.
中科院分区：
文献类型：
--
作者：
Cowieson, Nathan P.;Edwards-Gayle, Charlotte J. C.;Rambo, Robert P.
B21是英国Diamond Light Source Ltd同步加速器的3 GeV储存环中的小角X射线散射（SAXS）光束线。光束线利用双层单色器和环形聚焦光学器件，每秒将2 x 10（12）个光子传送到真空Eiger 4 M（Dectris）检测器处的34 x 40 μ m（FWHM）焦斑。高性能液相色谱系统和液体处理机器人可以将溶液样品加载到温度受控的真空样品池中，实现高度自动化。或者，可以使用一系列定制样品池手动加载一系列粘性或固体材料。默认散射矢量范围为0.0026至0.34埃（-1）和低仪器背景使B21便于测量各种生物大分子。自2013年以来，光束线已运行一个完整的用户程序。
B21 is a small-angle X-ray scattering (SAXS) beamline with a bending magnet source in the 3 GeV storage ring at the Diamond Light Source Ltd synchrotron in the UK. The beamline utilizes a double multi-layer monochromator and a toroidal focusing optic to deliver 2 x 10(12) photons per second to a 34 x 40 mu m (FWHM) focal spot at the in-vacuum Eiger 4M (Dectris) detector. A high-performance liquid chromatography system and a liquid-handling robot make it possible to load solution samples into a temperature-controlled in-vacuum sample cell with a high level of automation. Alternatively, a range of viscous or solid materials may be loaded manually using a range of custom sample cells. A default scattering vector range from 0.0026 to 0.34 angstrom(-1) and low instrument background make B21 convenient for measuring a wide range of biological macromolecules. The beamline has run a full user programme since 2013.