Pterygia pathogenesis - Corneal invasion by matrix metalloproteinase expressing altered limbal epithelial basal cells
Pterygia pathogenesis - Corneal invasion by matrix metalloproteinase expressing altered limbal epithelial basal cells
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DOI：
10.1001/archopht.119.5.695
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发表时间：
2001-05-01
影响因子：
--
通讯作者：
Reid, TW
中科院分区：
文献类型：
--
作者：
Dushku, N;John, MK;Reid, TW
目的：通过比较基质金属蛋白酶（MMPs）在改变的角膜缘基底干细胞、活化的成纤维细胞和翼状胬肉邻近的弹性变性区域中的免疫定位模式，评估MMPs在翼状胬肉发病机制中的潜在作用。9例原发性翼状胬肉和1例复发性翼状胬肉沿着正常上级角膜缘结膜组织和角膜，用MMP-1，MMP-2，结果：正常结膜、角膜缘和角膜细胞除细胞表面的MT 1-MMP外，其余均无明显免疫组化染色。与此相反，改变角膜缘基底上皮细胞的9个原发性和1个复发性翼状胬肉免疫染色的所有6个基质金属蛋白酶。与翼状胬肉相关的活化和改变的成纤维细胞主要对MMP-1进行免疫染色。相比之下，弹力变性（pingueculae）的基质领域表现出可变的MMPs.Conclusions免疫染色：改变角膜缘基底上皮细胞（翼状胬肉细胞）免疫染色的多种类型的MMPs在正常结膜，角膜缘，角膜细胞。侵袭Bowman层的翼状胬肉细胞产生升高的MMP-1、MMP-2和MMP-9表达，它们可能是负责Bowman层溶解的主要MMP。翼状胬肉细胞也可引起翼状胬肉头部成纤维细胞的活化，通过产生MMP-1导致Bowman层中纤维胶原的初始裂解。翼状胬肉后面的弹性组织变性区域（睑裂斑）中的改变的成纤维细胞构成第二种类型的肿瘤，其是非侵入性的。临床相关性：这些改变的MMP表达的数据支持改变的基底角膜缘上皮细胞在翼状胬肉的形成和迁移中起关键作用的概念。
Objective: To assess the potential role of matrix metalloproteinases (MMPs) in the pathogenesis of pterygia by comparing the immunolocalization patterns of MMPs in altered limbal basal stem cells, activated fibroblasts, and areas of elastotic degeneration adjacent to the pterygia.Methods: Nine primary and 1 recurrent pterygia along with normal superior limbal-conjunctival tissue and cornea were immunostained with mouse monoclonal antibodies specific for MMP-1, MMP-2, MMP-3, MMP-9, membrane type 1 (MT1)-MMP (MMP-14), and membrane type 2-MMP (MMP-15).Results: Normal conjunctival, limbal, and corneal cells lacked significant immunostaining except for cell surface MT1-MMP. In contrast, altered limbal basal epithelial cells of the 9 primary and 1 recurrent pterygia immunostained for all 6 MMPs. Activated and altered fibroblasts associated with the pterygia immunostained primarily for MMP-1. In contrast, stromal areas of elastotic degeneration (pingueculae) showed variable immunostaining of MMPs.Conclusions: Altered limbal basal epithelial cells (pterygium cells) immunostained for multiple types of MMPs in contrast to normal conjunctival, limbal, and corneal cells. The pterygium cells invading over Bowman's layer produce elevated MMP-1, MMP-2, and MMP-9 expression, which probably are the main MMPs responsible for the dissolution of Bowmen's layer. Pterygium cells may also cause activation of fibroblasts at the head of the pterygium, leading to the initial cleavage of fibrillar collagen in Bowman's layer by the production of MMP-1. Altered fibroblasts in areas of elastotic degeneration (pingueculae) trailing behind the pterygium constitute a second type of tumor, which is noninvasive.Clinical Relevance: These data of altered MMP expression support the concept that altered basal limbal epithelial cells play a key role in the formation and migration of a pterygium.