A comparison of fibrin, agarose and gellan gum hydrogels as carriers of stem cells and growth factor delivery microspheres for cartilage regeneration
A comparison of fibrin, agarose and gellan gum hydrogels as carriers of stem cells and growth factor delivery microspheres for cartilage regeneration
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DOI：
10.1088/1748-6041/8/3/035004
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发表时间：
2013-06-01
影响因子：
4
通讯作者：
Kelly, Daniel J.
中科院分区：
文献类型：
--
作者：
Ahearne, Mark;Kelly, Daniel J.
关节软骨有限的内在修复能力导致了对不同治疗方案的研究，以促进其再生。含有干细胞或祖细胞和生长因子释放微球的水凝胶的递送代表了软骨修复的有吸引力的方法。在这项研究中，研究了包封水凝胶对祖细胞与TGF-β 3释放微球偶联形成软骨组织的能力的影响。在化学成分确定的培养基中培养含有TGF-β 3负载的明胶微球和源自膝盖的髌下脂肪垫的祖细胞的明胶、琼脂糖和结冷胶水凝胶21天。在TGF-β 3释放微球的存在下，观察到结冷胶水凝胶比纤维蛋白或琼脂糖水凝胶促进更大的细胞增殖。水凝胶的组织学和生物化学分析表明，纤维蛋白是三种水凝胶中软骨诱导性最小的，而琼脂糖和结冷胶似乎支持更稳健的软骨形成，如这些构建体内更大的sGAG积累所证明的。结冷胶水凝胶对II型胶原和I型胶原的染色也更强烈，这表明尽管这些构建体中的总胶原合成更高，但表型可能在性质上比正常透明软骨更具纤维软骨性。这项研究表明，当将包封水凝胶掺入生长因子递送系统时，它如何对干细胞形成软骨的能力产生显着影响。
The limited intrinsic repair capacity of articular cartilage has led to the investigation of different treatment options to promote its regeneration. The delivery of hydrogels containing stem or progenitor cells and growth factor releasing microspheres represents an attractive approach to cartilage repair. In this study, the influence of the encapsulating hydrogel on the ability of progenitor cells coupled with TGF-beta 3 releasing microspheres to form cartilaginous tissue was investigated. Fibrin, agarose and gellan gum hydrogels containing TGF-beta 3 loaded gelatin microspheres and progenitor cells derived from the infrapatellar fat-pad of the knee were cultured for 21 days in a chemically defined media. In the presence of TGF-beta 3 releasing microspheres, gellan gum hydrogels were observed to facilitate greater cell proliferation than fibrin or agarose hydrogels. Histological and biochemical analysis of the hydrogels indicated that fibrin was the least chondro-inductive of the three hydrogels, while agarose and gellan gum appeared to support more robust cartilage formation as demonstrated by greater sGAG accumulation within these constructs. Gellan gum hydrogels also stained more intensely for collagen type II and collagen type I, suggesting that although total collagen synthesis was higher in these constructs, that the phenotype may be more fibrocartilaginous in nature than normal hyaline cartilage. This study demonstrates how the encapsulating hydrogel can have a significant impact on the ability of stem cells to form cartilage when incorporated into a growth factor delivery system.