Bluetongue virus isolations from midges belonging to the Obsoletus complex (Culicoides, Diptera: ceratopogonidae) in Italy
Bluetongue virus isolations from midges belonging to the Obsoletus complex (Culicoides, Diptera: ceratopogonidae) in Italy
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DOI：
10.1136/vr.157.5.133
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发表时间：
2005-07-30
影响因子：
2.2
通讯作者：
Caporale, V
中科院分区：
文献类型：
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作者：
Savini, G;Goffredo, M;Caporale, V
2002年7月至9月期间，在San Gregorio Magno（坎帕尼亚萨莱诺）、拉维亚诺（坎帕尼亚萨莱诺）和Carpino（福贾，普利亚）社区的三个绵羊饲养场爆发了蓝舌病，血清学和病毒学证实了蓝舌病病毒（BTV）的参与。死亡率至少为11%，涉及BTV血清2型（BTV-2）和血清9型（BTV-9）。还调查了这些藏品中的库蠓（双翅目：蠓科）媒介;捕获了属于15个物种的约10 000只蠓，但其中不包括经典的亚非蓝舌病媒介，拟态库蠓的单一标本。属于过时的复杂的物种占主导地位的光陷阱收集，和库蠓过时的Meigen，库蠓scoticus唐斯和水壶和库蠓dewulfi Goetghebuer构成了90%的所有库蠓物种捕获。对五十六池的Obsoletus复合群（不包括德武菲库蚊）进行病毒检测，每个池含有100只个体蠓，仅含有经产和妊娠雌蚊。BTV-2从San Gregorio Magno和Carpino的三个池中分离，BTV-9从拉维亚诺的一个池中分离。这些结果表明，一个物种以外的C imicola参与目前重新出现的蓝舌病在地中海盆地，但无论是C obsoletus狭义或C scoticus，或两者兼而有之，是不确定的。
Between July and September 2002 there were outbreaks of bluetongue on three sheep holdings in the communities of San Gregorio Magno (Salerno, Campania), Laviano (Salerno, Campania) and Carpino (Foggia, Puglia), and the involvement of bluetongue virus (BTV) was confirmed serologically and virologically. The mortality rate was at least 11 per cent and involved BTV serotype 2 (BTV-2) and serotype 9 (BTV-9). These holdings were also surveyed for the Culicoides (Diptera: Ceratopogonidae) vectors; approximately 10,000 midges belonging to 15 species were captured, but they did not include a single specimen of the classical Afro-Asiatic bluetongue vector, Culicoides imicola. Species belonging to the obsoletus complex dominated the light-trap collections, and Culicoides obsoletus Meigen, Culicoides scoticus Downes and Kettle and Culicoides dewulfi Goetghebuer constituted 90 per cent of all the Culicoides species captured. Fifty-six pools of the Obsoletus complex (excluding C dewuffi), each containing 100 individual midges and containing only parous and gravid females, were assayed for virus. BTV-2 was isolated from three pools from San Gregorio Magno and Carpino, and BTV-9 was isolated from one pool from Laviano. These results indicate that a species other than C imicola is involved in the current re-emergence of bluetongue in the Mediterranean Basin, but whether it is C obsoletus sensu stricto or C scoticus, or both, is uncertain.