Saccharothrix deserti sp. nov., an actinomycete isolated from desert soil
Saccharothrix deserti sp. nov., an actinomycete isolated from desert soil
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沙漠糖丝菌
DOI：
10.1099/ijsem.0.003989
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发表时间：
--
影响因子：
2.8
通讯作者：
Jihong Jiang
中科院分区：
文献类型：
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作者：
Jinjuan Liu;Yong Sun;Jinrong Liu;Yunjian Wu;Chengliang Cao;Rongpeng Li;Jihong Jiang
从中国西北部新疆省的沙漠土壤中分离出一种革兰氏染色阳性的需氧放线菌，命名为菌株 BMP B8144T。该分离物在大多数测试培养基上产生很少的气生菌丝体和破碎的基质菌丝体。细胞壁水解产物含有内消旋二氨基庚二酸、半乳糖和甘露糖。诊断磷脂是二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰羟基乙醇胺、磷脂酰肌醇和磷脂酰肌醇甘露糖苷。主要脂肪酸为iso-C16 : 0、C17 : 1ω8candiso-C15 : 0。主要的甲基萘醌为MK-9(H4)和MK-10(H4)。 DNA G+C 含量为70.4 mol％(基因组)。根据EzBioCloud服务器上的16S rRNA基因序列分析，菌株BMP B8144T与Saccharothrix lopnurensisYIM LPA2hT（98.9%）和‘Saccharothrix yanglingensis’Hhs.015（98.6 %）最相似。然而，根据低水平的 DNA-DNA 相关性（分别为 59.3±1.8% 和 47.9±2.3%），它可以与最接近的菌株区分开来。菌株BMP B8144T结合了形态学、化学分类学和系统发育特征，代表了糖丝菌属的一个新种，其名称为Saccharothrix Desertisp。十一月被提议。模式菌株是BMP B8144T(=CGMCC 4.7490T=KCTC 49001T)。
A Gram-stain-positive, aerobic actinomycete, designated strain BMP B8144T, was isolated from desert soil, in Xinjiang province, northwest China. The isolate produced scanty aerial mycelium and fragmented substrate mycelium on most tested media. Cell-wall hydrolysates containedmeso-diaminopimelic acid, galactose and mannose. The diagnostic phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylhydroxylethanolamine, phosphatidylinositol, and phosphatidylinositol mannosides. The major fatty acids includediso-C16 : 0, C17 : 1ω8candiso-C15 : 0. The predominant menaquinones were MK-9(H4) and MK-10(H4). The DNA G+C content was 70.4 mol% (genome). Based on the 16S rRNA gene sequence analysis on EzBioCloud server, strain BMP B8144Tshowed the closest similarities toSaccharothrix lopnurensisYIM LPA2hT(98.9 %) and ‘Saccharothrix yanglingensis’ Hhs.015 (98.6 %). However, it can be distinguished from the closest strains based on the low levels of DNA–DNA relatedness (59.3±1.8 and 47.9±2.3 %, respectively). A combination of morphological, chemotaxonomic and phylogenetic characteristics, strain BMP B8144Trepresents a novel species of the genusSaccharothrix, for which the nameSaccharothrix desertisp. nov. is proposed. The type strain is BMP B8144T(=CGMCC 4.7490T=KCTC 49001T).