Effect of Brn-3a deficiency on parvalbumin-immunoreactive primary sensory neurons in the dorsal root ganglion
Effect of Brn-3a deficiency on parvalbumin-immunoreactive primary sensory neurons in the dorsal root ganglion
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DOI：
10.1016/j.devbrainres.2004.02.004
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发表时间：
2004-05-19
期刊：
影响因子：
--
通讯作者：
Sugimoto, T
中科院分区：
文献类型：
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作者：
Ichikawa, H;Mo, Z;Sugimoto, T
分别于出生后第0天和胚胎第18.5天在野生型和基因敲除小鼠背根神经节(DRG)进行BRN-3a的免疫组织化学染色。野生型(5.4%)和基因敲除小鼠(5.6%)背根神经节含有大量的小白蛋白免疫反应(Ir)神经元。细胞大小分析表明，这些小鼠的这种神经元大多是中型到大型的。因此，本体感受器的生存不太可能依赖于DRG中的BRN-3a。然而，在基因敲除小鼠的脊髓背柱和灰质中，与野生型小鼠相比，小白蛋白免疫反应神经纤维稀疏。突变体中运动神经元周围的小白蛋白免疫反应曲张的数量减少。因此，我们的数据提示，BRN-3a可能在脊髓DRG本体感受器的中枢投射和终末形成中发挥重要作用。(C)2004爱思唯尔B.V.保留所有权利。
Immunohistochemistry for parvalbumin, a marker for primary proprioceptors, was performed on the dorsal root ganglion (DRG) of wildtype and knockout mice for Brn-3a at postnatal day 0 and embryonic day 18.5. The DRG contained many parvalbumin-immunoreactive (ir) neurons in wildtype (5.4%) and knockout mice (5.6%). Cell size analysis demonstrated that such neurons were mostly medium-sized to large in these mice. Therefore, it is unlikely that the survival of proprioceptors is dependent upon Brn-3a in the DRG. In the dorsal column and gray matter of the spinal cord of knockout mice, however, parvalbumin-ir nerve fibers were sparse compared to wildtype mice. The number of parvalbumin-ir varicosities around motoneurons decreased in the mutant. Thus, our data suggest that Brn-3a may play an important role in the central projection and terminal formation of DRG proprioceptors in the spinal cord. (C) 2004 Elsevier B.V. All rights reserved.