High Resolution Electroencephalography in Freely Moving Mice
High Resolution Electroencephalography in Freely Moving Mice
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DOI：
10.1152/jn.00188.2010
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发表时间：
2010-09-01
影响因子：
2.5
通讯作者：
Shin, Hee-Sup
中科院分区：
文献类型：
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作者：
Choi, Jee Hyun;Koch, Klaus Peter;Shin, Hee-Sup
Choi JH，Koch KP，Poppendieck W，Lee M，Shin HS.自由活动小鼠的高分辨率脑电图。J Neurophysiol 104：1825-1834，2010.首次发表于2010年7月7日; doi：10.1152/jn.00188.2010。脑电图（EEG）是用于监测人类大脑状态的标准工具。通过使用分子、药理学或电生理学操作下的小鼠模型，可以促进理解不同EEG节律的分子和细胞机制。然而，小鼠大脑的小尺寸对EEG的空间信息造成了严重的限制。为了克服这一限制，我们设计了一个基于聚酰亚胺的微电极阵列（PBM阵列）与纳米纤维技术。微电极包含32个电极，重150毫克，并产生噪声不敏感的信号时，应用于小鼠头骨。高密度微电极允许在小鼠大脑中进行高分辨率EEG（HR-EEG）的全局和聚焦映射。映射和动态分析工具也已被开发，以可视化的空间分辨小鼠脑电的动态变化。我们证明了小鼠EEG在转基因小鼠失神发作模型和异常θ节律的相位动力学定位中的有效性和实用性。在自由移动的条件下，在转基因小鼠的EEG图的动态跟踪应该允许的分子和细胞机制的基础上的不同的EEG节律的生成和动态的研究。
Choi JH, Koch KP, Poppendieck W, Lee M, Shin HS. High resolution electroencephalography in freely moving mice. J Neurophysiol 104: 1825-1834, 2010. First published July 7, 2010; doi: 10.1152/jn.00188.2010. Electroencephalography (EEG) is a standard tool for monitoring brain states in humans. Understanding the molecular and cellular mechanisms underlying diverse EEG rhythms can be facilitated by using mouse models under molecular, pharmacological, or electrophysiological manipulations. The small size of the mouse brain, however, poses a severe limitation in the spatial information of EEG. To overcome this limitation, we devised a polyimide based microelectrode array (PBM array) with nanofabrication technologies. The microelectrode contains 32 electrodes, weighs 150 mg, and yields noise-insensitive signals when applied on the mouse skull. The high-density microelectrode allowed both global and focused mapping of high resolution EEG (HR-EEG) in the mouse brain. Mapping and dynamical analysis tools also have been developed to visualize the dynamical changes of spatially resolved mouse EEG. We demonstrated the validity and utility of mouse EEG in localization of the seizure onset in absence seizure model and phase dynamics of abnormal theta rhythm in transgenic mice. Dynamic tracking of the EEG map in genetically modified mice under freely moving conditions should allow study of the molecular and cellular mechanisms underlying the generation and dynamics of diverse EEG rhythms.