Acetylome analysis reveals the involvement of lysine acetylation in biosynthesis of antibiotics in Bacillus amyloliquefaciens.
Acetylome analysis reveals the involvement of lysine acetylation in biosynthesis of antibiotics in Bacillus amyloliquefaciens.
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乙酰组分析揭示赖氨酸乙酰化参与解淀粉芽孢杆菌抗生素的生物合成
DOI：
10.1038/srep20108
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发表时间：
2016-01-29
影响因子：
4.6
通讯作者：
Liang W
中科院分区：
文献类型：
--
作者：
Liu L;Wang G;Song L;Lv B;Liang W
赖氨酸乙酰化是一种重要的翻译后修饰，在真核生物和原核生物中几乎在各个方面都发挥着重要的调节作用。淀粉解性芽孢杆菌是一种革兰氏阳性菌，对植物病原菌的防治非常有效。然而，人们对赖氨酸乙酰化在该生物体中的作用知之甚少。在这里，我们通过高灵敏的免疫亲和纯化和高分辨LC−MS/MS相结合的方法对淀粉液化芽孢杆菌进行了首次赖氨酸乙酰化组分的研究，共鉴定了1254个蛋白质中的3268个赖氨酸乙酰化位点，占该细菌总蛋白质的32.9%。到目前为止，这是在细菌中发现的乙酰化蛋白质的最高比例。乙酰化蛋白质与多种生物过程有关，其中很大一部分参与新陈代谢。有趣的是，我们首次发现与三种类型的多肽和五类脂肽的合成密切相关的所有蛋白质中，分别有71.1%(27/38)和78.6%(22/28)被乙酰化。这些发现表明，赖氨酸乙酰化在调节抗生素的生物合成中起着关键作用。这些数据为进一步阐明赖氨酸乙酰化在淀粉液化芽孢杆菌中的生理作用提供了重要资源。
Lysine acetylation is a major post-translational modification that plays an important regulatory role in almost every aspects in both eukaryotes and prokaryotes. Bacillus amyloliquefaciens, a Gram-positive bacterium, is very effective for the control of plant pathogens. However, very little is known about the function of lysine acetylation in this organism. Here, we conducted the first lysine acetylome in B. amyloliquefaciens through a combination of highly sensitive immune-affinity purification and high-resolution LC−MS/MS. Overall, we identified 3268 lysine acetylation sites in 1254 proteins, which account for 32.9% of the total proteins in this bacterium. Till date, this is the highest ratio of acetylated proteins that have been identified in bacteria. Acetylated proteins are associated with a variety of biological processes and a large fraction of these proteins are involved in metabolism. Interestingly, for the first time, we found that about 71.1% (27/38) and 78.6% (22/28) of all the proteins tightly related to the synthesis of three types of pepketides and five families of lipopeptides were acetylated, respectively. These findings suggest that lysine acetylation plays a critical role in the regulation of antibiotics biosynthesis. These data serves as an important resource for further elucidation of the physiological role of lysine acetylation in B. amyloliquefaciens.