Selective resonance suppression 1H-[13C] NMR spectroscopy with asymmetric adiabatic RF pulses.
Selective resonance suppression 1H-[13C] NMR spectroscopy with asymmetric adiabatic RF pulses.
复制标题
具有不对称绝热射频脉冲的选择性共振抑制 1H-[13C] NMR 波谱。
DOI：
10.1002/mrm.21829
复制
发表时间：
2009
影响因子：
3.3
通讯作者：
Gruetter,Rolf
中科院分区：
文献类型：
--
作者：
Xin,Lijing;Frenkel,Hanne;Mlynárik,Vladimír;Morgenthaler,FlorenceD;Gruetter,Rolf
Despite obvious improvements in spectral resolution at high magnetic field, the detection of13C labeling by1H‐[13C] NMR spectroscopy remains hampered by spectral overlap, such as in the spectral region of1H resonances bound to C3 of glutamate (Glu) and glutamine (Gln), and C6 of N‐acetylaspartate (NAA). The aim of this study was to develop, implement, and apply a novel1H‐[13C] NMR spectroscopic editing scheme, dubbed “selective Resonance suppression by Adiabatic Carbon Editing and Decoupling single‐voxel STimulated Echo Acquisition Mode” (RACED‐STEAM). The sequence is based on the application of two asymmetric narrow‐transition‐band adiabatic RF inversion pulses at the resonance frequency of the13C coupled to the protons that need to be suppressed during the mixing time (TM) period, alternating the inversion band downfield and upfield from the13C resonance on odd and even scans, respectively, thus suppressing the detection of1H resonances bound to13C within the transition band of the inversion pulse. The results demonstrate the efficient suppression of1H resonances bound to C3 of Glu and Gln, and C4 of Glu, which allows the1H resonances bound to C6 of NAA and C4 of Gln to be revealed. The measured time course of the resolved labeling into NAA C6 with the new scheme was consistent with the slow turnover of NAA. Magn Reson Med 61:260–266, 2009. © 2009 Wiley‐Liss, Inc.
