CHARACTERIZATION OF INTERSTITIAL STEM-CELLS IN HYDRA BY CLONING
CHARACTERIZATION OF INTERSTITIAL STEM-CELLS IN HYDRA BY CLONING
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DOI：
10.1016/0012-1606(77)90098-7
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发表时间：
1977-01-01
影响因子：
2.7
通讯作者：
MURPHY, S
中科院分区：
文献类型：
--
作者：
DAVID, CN;MURPHY, S
A procedure was developed for cloning interstitial stem cells from hydra. Clones were prepared by introducing small numbers of viable cells into aggregates of nitrogen mustard-inactivated host tissue. Clones derived from added stem cells were identified after 1-2 wk of growth by staining with toluidine blue. The incidence of clones increased with increasing input of viable cells according to 1-hit Poisson statistics, indicating that clones arose from single cells. After correction for cell losses in the procedure, .apprx. 1.2% of the input cells formed clones. This compared with estimates from in vivo experiments of .apprx. 4% stem cells in whole hydra (David and Gierer, 1974). Differentiation of nematocytes and nerve cells in clones was analyzed by labeling precursors with [3H]thymidine and scoring labeled nerves and nematocytes 2 days later. Clones (9) examined in this way contained differentiated nerve cells and nematocytes, demonstrating that the interstitial stem cell was multipotent. The localization of nerve and nematocyte differentiation in whole hydra probably occurred at the level of stem-cell determination. Differentiated cells occurring very early in clone development suggests that a stem cell''s decision to proliferate or differentiate is regulated by short-range feedback signals already saturated in young clones.
