Integrated device for combined optical neuromodulation and electrical recording for chronic in vivo applications
Integrated device for combined optical neuromodulation and electrical recording for chronic in vivo applications
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DOI：
10.1088/1741-2560/9/1/016001
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发表时间：
2012-02-01
影响因子：
4
通讯作者：
Deisseroth, Karl
中科院分区：
文献类型：
--
作者：
Wang, Jing;Wagner, Fabien;Deisseroth, Karl
研究大脑功能及其局部回路动力学需要能够以高时空分辨率记录和刺激大脑的神经接口。光遗传学是一种基因靶向特定神经元以表达光敏通道蛋白的技术，提供了以毫秒级时间精度控制哺乳动物中枢神经系统神经元活动的能力。该技术能够对神经元进行精确的光学刺激，并通过电生理手段同时监测刺激部位附近和远处的神经反应。我们以前证明，在体外，双重能力（光传输和电记录），同时测试一种新的混合装置（optrode-MEA），其中包括一个锥形同轴光学电极（optrode）和100元素微电极阵列（MEA）。在这里，我们报告了一个完全慢性植入的新版本的这种设备在ChR 2表达的大鼠，并证明其使用在自由活动的动物在长达8个月的时间。在其目前的配置中，我们展示了将光学激励传递到单个皮质部位的设备，同时映射来自6 x 6元件MEA的周围30个通道的神经响应，从而能够记录跨几毫米的新皮质景观的光学调制的单个单元和局部场电位活动。
Studying brain function and its local circuit dynamics requires neural interfaces that can record and stimulate the brain with high spatiotemporal resolution. Optogenetics, a technique that genetically targets specific neurons to express light-sensitive channel proteins, provides the capability to control central nervous system neuronal activity in mammals with millisecond time precision. This technique enables precise optical stimulation of neurons and simultaneous monitoring of neural response by electrophysiological means, both in the vicinity of and distant to the stimulation site. We previously demonstrated, in vitro, the dual capability (optical delivery and electrical recording) while testing a novel hybrid device (optrode-MEA), which incorporates a tapered coaxial optical electrode (optrode) and a 100 element microelectrode array (MEA). Here we report a fully chronic implant of a new version of this device in ChR2-expressing rats, and demonstrate its use in freely moving animals over periods up to 8 months. In its present configuration, we show the device delivering optical excitation to a single cortical site while mapping the neural response from the surrounding 30 channels of the 6 x 6 element MEA, thereby enabling recording of optically modulated single-unit and local field potential activity across several millimeters of the neocortical landscape.