Hyperbaric oxygen inhibits stimulus-induced proinflammatory cytokine synthesis by human blood-derived monocyte-macrophages
Hyperbaric oxygen inhibits stimulus-induced proinflammatory cytokine synthesis by human blood-derived monocyte-macrophages
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DOI：
10.1046/j.1365-2249.2003.02248.x
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发表时间：
2003-10-01
影响因子：
4.6
通讯作者：
Granowitz, EV
中科院分区：
文献类型：
--
作者：
Benson, RM;Minter, LM;Granowitz, EV
高压氧（HBO）是在升高的大气压下向患有炎症性疾病的患者提供100%氧气。我们建立了一个体外模型来研究HBO对刺激诱导的促炎细胞因子转录和翻译的影响。在转移到HBO室之前刺激人血液来源的单核细胞-巨噬细胞，在HBO室中将它们在97.9%O-2、2.1%CO2、2.4个绝对大气压、37 ℃下孵育。对照组保持在同一温暖的房间中，在海平面正常氧，高氧或单独增加的压力。一个90分钟的HBO暴露抑制IL-1 β合成响应脂多糖的23%，脂质A的45%，植物血凝素A（PHA）的68%，和肿瘤坏死因子（TNF）-α的27%。HBO抑制脂多糖，脂质A和PHA诱导的TNF-α分别为29%，31%和62%。HBO短暂降低PHA诱导的稳态IL-1 β mRNA水平。单独的高氧和单独的压力不影响细胞因子的产生。HBO对单核-巨噬细胞的免疫抑制作用超过3 h后不再明显。有趣的是，暴露于HBO 12小时的细胞比对照条件下培养的细胞合成更多的IL-1 β。总之，HBO暴露短暂抑制刺激诱导的促炎细胞因子的产生和稳态RNA水平。
Hyperbaric oxygen (HBO) is 100% oxygen administered at elevated atmospheric pressure to patients with inflammatory diseases. We developed an in vitro model to investigate the effects of HBO on stimulus-induced proinflammatory cytokine transcription and translation. Human blood-derived monocyte-macrophages were stimulated before being transferred to an HBO chamber where they were incubated at 97.9% O-2, 2.1% CO2, 2.4 atmospheres absolute, 37degreesC. Controls were maintained in the same warm room at normoxia at sea level, hyperoxia or increased pressure alone. A 90-min HBO exposure inhibited IL-1beta synthesized in response to lipopolysaccharide by 23%, lipid A by 45%, phytohaemagglutinin A ( PHA) by 68%, and tumour necrosis factor (TNF)-alpha by 27%. HBO suppressed lipopolysaccharide-, lipid A- and PHA-induced TNF-alpha by 29%, 31% and 62%, respectively. HBO transiently reduced PHA-induced steady state IL-1beta mRNA levels. Hyperoxia alone and pressure alone did not affect cytokine production. The immunosuppressive effect of HBO was no longer evident in monocyte-macrophages exposed to HBO for more than 3 h. Interestingly, cells exposed to HBO for 12 h synthesized more IL-1beta than cells cultured under control conditions. In summary, HBO exposure transiently suppresses stimulus-induced proinflammatory cytokine production and steady state RNA levels.