Lymphocyte transformation induced by autologous cells. III. Lymphoblast-induced lymphocyte to stimulation does not correlate with EB viral antigen expression or immunity.
Lymphocyte transformation induced by autologous cells. III. Lymphoblast-induced lymphocyte to stimulation does not correlate with EB viral antigen expression or immunity.
复制标题
自体细胞诱导淋巴细胞转化。
作者：
M. Weksler
人丝裂原诱导的和细胞系B淋巴母细胞刺激培养物中同种异体和自体淋巴细胞的增殖。本文研究了EB病毒决定簇在刺激细胞反应和淋巴细胞供体对EB病毒的免疫反应中的作用。将淋巴母细胞与EB病毒决定簇的抗血清一起孵育不会抑制培养细胞系淋巴母细胞的刺激能力。培养的细胞系B成淋巴细胞刺激来自具有或不具有EB病毒免疫力的供体的淋巴细胞掺入同样多的胸苷。此外，缺乏EB病毒基因组的B淋巴母细胞系（U-698）与具有EB基因组的B淋巴母细胞一样刺激淋巴细胞增殖。培养的T淋巴母细胞系不刺激同种异体淋巴细胞增殖。这些细胞似乎缺乏刺激淋巴细胞转化的决定因子。没有证据表明培养的T淋巴母细胞抑制同种异体淋巴细胞增殖。丝裂原诱导的EB免疫和非免疫受试者的淋巴母细胞刺激自体淋巴细胞增殖。结论：对EB病毒的免疫和EB病毒抗原在有丝分裂原诱导的淋巴母细胞系上的表达对于刺激淋巴细胞增殖都是不必要的。
Human mitogen-induced and cell line B lymphoblasts stimulate the proliferation of allogeneic and autologous lymphocytes in culture. The role in thes reaction of EB viral determinants on the stimulating cells and immunity of the lymphocyte donor to the EB virus has been studied. The stimulatory capacity of cultured cell line lymphoblasts is not inhibited by incubating lymphoblasts with antisera to EB viral determinants. Cultured cell line B lymphoblasts stimulate as much thymidine incorporation by lymphocytes from donors with or without immunity to the EB virus. Further, a B lymphoblast cell line (U-698) which lacks the EB viral genome stimulated as much lymphocyte proliferation as did B lymphoblasts with the EB genome. Cultured T lymphoblast cell lines do not stimulate allogeneic lymphocyte proliferation. These cells appear to lack the determinants which stimulate lymphocyte transformation. No evidence was found that cultured cell line T lymphoblasts suppressed allogeneic lymphocyte proliferation. Mitogeninduced lymphoblasts from EB-immune and non-immune subjects stimulated the proliferation of autologous lymphocytes comparably. It is concluded that neither immunity to the EB virus nor expression of EB viral antigens on mitogen-induced on cell line lymphoblasts is necessary for the stimulation of lymphocyte proliferation.