Gene expression and activity of antioxidant enzymes in barley (Hordeum vulgare L.) under controlled severe drought
Gene expression and activity of antioxidant enzymes in barley (Hordeum vulgare L.) under controlled severe drought
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DOI：
10.1080/17429145.2015.1033023
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发表时间：
2015-01-01
影响因子：
3.2
通讯作者：
Samarah, Nezar
中科院分区：
文献类型：
--
作者：
Harb, Amal;Awad, Dalal;Samarah, Nezar
本研究旨在测定两种大麦基因型在干旱条件下超氧化物歧化酶（SOD）、抗坏血酸过氧化物酶（APX）和过氧化氢酶（CAT）的活性及其基因表达。为了实现这一目标，将朗姆酒和耶尔穆克两种基因型的21日龄大麦植株暴露在受控的严重干旱（25%的田间容量）中2天、9天和16天。干旱处理2 d后，朗姆酒基因型植株超氧化物歧化酶活性显著升高。在Yarmouk基因型中，干旱处理2 d和9 d后APX活性显著升高。在Rum基因型中，CAT2在干旱处理9天后上调，SOD和APX在干旱处理16天后上调，而在Yarmouk基因型中，CAT2、SOD和APX在干旱处理2天后上调。结果表明，两种大麦基因型抗氧化酶在抗旱条件下具有独特的活性和基因表达模式。此外，数据表明，在相同的干旱条件下，每个基因型利用不同的分子和生化反应。
The objective of the present study was to determine the activity of antioxidant enzymes: superoxide dismutase (SOD), ascorbate peroxidase (APX), and catalase (CAT) and the expression of their genes in two barley genotypes under controlled severe drought. To fulfill this objective, 21-day-old barley plants of two genotypes: Rum and Yarmouk were exposed to controlled severe drought (25% field capacity) for 2, 9, and 16 days. The activity of SOD was significantly high in Rum genotype after 2 days of drought treatment. In Yarmouk genotype, the activity of APX was significantly high after 2 and 9 days of drought treatment. In Rum genotype, CAT2 was upregulated after 9 days of drought treatment and SOD and APX were upregulated after 16 days of drought treatment, whereas CAT2, SOD, and APX were upregulated in Yarmouk genotype after 2 days of drought treatment. The results indicate a unique pattern of activity and gene expression of the antioxidant enzymes in the two barley genotypes under controlled severe drought. Moreover, the data suggest that each genotype utilizes different molecular and biochemical responses under the same drought conditions.