Candidate EDA targets revealed by expression profiling of primary keratinocytes from Tabby mutant mice.
Candidate EDA targets revealed by expression profiling of primary keratinocytes from Tabby mutant mice.
复制标题
DOI：
10.1016/j.gene.2008.09.014
复制
发表时间：
2008-12-31
期刊：
影响因子：
3.5
通讯作者：
Schlessinger, David
中科院分区：
文献类型：
--
作者：
Esibizione, Diana;Cui, Chang-Yi;Schlessinger, David
EDA, the gene mutated in anhidrotic ectodermal dysplasia, encodes ectodysplasin, a TNF superfamily member that activates NF-kB mediated transcription. To identify EDA target genes, we have earlier used expression profiling to infer genes differentially expressed at various developmental time points in Tabby (Eda-deficient) compared to wild-type mouse skin. To increase the resolution to find genes whose expression may be restricted to epidermal cells, we have now extended studies to primary keratinocyte cultures established from E19 wild-type and Tabby skin. Using microarrays bearing 44,000 gene probes, we found 385 preliminary candidate genes whose expression was significantly affected by Eda loss. By comparing expression profiles to those from Eda-A1 transgenic skin, we restricted the list to 38 “candidate EDA targets”, 14 of which were already known to be expressed in hair follicles or epidermis. We confirmed expression changes for 3 selected genes, Tbx1, Bmp7, and Jag1, both in keratinocytes and in whole skin, by Q-PCR and Western blotting analyses. Thus, by the analysis of keratinocytes, novel candidate pathways downstream of EDA were detected.
登录
查看更多内容
影响因子：
30.8
作者：
Headon, DJ;Overbeek, PA
通讯作者：
Overbeek, PA
DOI：
10.1073/pnas.0509678103
发表时间：
2006-06-13
影响因子：
11.1
作者：
Cui, Chang-Yi;Hashimoto, Tsuyoshi;Schiessinger, David
通讯作者：
Schiessinger, David
影响因子：
3.5
作者：
Cui, CY;Durmowicz, M;Schlessinger, D
通讯作者：
Schlessinger, D
影响因子：
3.6
作者：
Kim, Hyun-Jin;Choi, Chun-Pill;Lee, Mu-Hyoung
通讯作者：
Lee, Mu-Hyoung
影响因子：
7
作者：
Carter, MG;Hamatani, T;Ko, MSH
通讯作者：
Ko, MSH