"Prompt" heat shock proteins: translationally regulated synthesis of new proteins associated with the nuclear matrix-intermediate filaments as an early response to heat shock.
"Prompt" heat shock proteins: translationally regulated synthesis of new proteins associated with the nuclear matrix-intermediate filaments as an early response to heat shock.
复制标题
“迅速”热休克蛋白：与核基质中间丝相关的新蛋白质的翻译调节合成，作为对热休克的早期反应。
DOI：
10.1073/pnas.80.15.4737
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发表时间：
1983
影响因子：
11.1
通讯作者：
Penman,S
中科院分区：
文献类型：
--
作者：
Reiter,T;Penman,S
哺乳动物细胞，如HeLa细胞，对延长暴露于升高的温度（称为热休克）的反应已得到很好的表征。在这些研究中，几种蛋白质合成了新的mRNA，这些蛋白质的翻译在回到37摄氏度后最好。我们在这里展示了对温度升高的另一种反应，其性质截然不同。一组至少50个新的可检测的蛋白质，专门与核基质-中间丝（NM-IF）部分，是立即合成暴露于高温。这些是非常低的丰度或不存在的非应激细胞，似乎没有对应于新的转录后产生的“经典”热休克蛋白。用放线菌素D预先处理对这些“提示”蛋白几乎没有影响，它们似乎是由在升高的温度下激活的预先存在的mRNA制成的。在可溶性，细胞骨架，和染色质组分的蛋白质合成强烈减少的温度升高，而与NM-IF复合物迅速上升几倍以上的控制细胞的提示蛋白的标记。此外，这些结果表明，这四个细胞组分不是任意的细胞分裂;相反，它们代表了细胞中生理上重要的隔室。
The response of mammalian cells, such as HeLa cells, to prolong exposure to increased temperature (termed heat shock) has been well characterized. In these studies new mRNA is synthesized for several proteins whose translation is best seen after a return to 37 degrees C. We show here another response to increased temperature of a distinctively different character. A set of at least 50 newly detectable proteins, exclusively associated with the nuclear matrix-intermediate filaments (NM-IF) fraction, is synthesized immediately upon exposure to high temperature. These are of very low abundance or nonexistent in the unstressed cell and none appear to correspond to the "classic" heat shock proteins produced after new transcription. Prior treatment with actinomycin D has little effect on these "prompt" proteins, and they appear to be made from preexisting mRNAs that are activated at the increased temperature. The protein synthesis in the soluble, cytoskeletal, and chromatin fractions is strongly reduced by the increased temperature, while the labeling of the prompt proteins associated with NM-IF complex rapidly rises severalfold above that in control cells. Additionally these results suggest that the four cell fractions are not arbitrary cell divisions; rather they represent physiologically significant compartments in the cell.