Analysis of deletion mutations of the rpsL gene in the yeast Saccharomyces cerevisiae detected after long-term flight on the Russian space station Mir
Analysis of deletion mutations of the rpsL gene in the yeast Saccharomyces cerevisiae detected after long-term flight on the Russian space station Mir
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DOI：
10.1016/s1383-5742(00)00054-5
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发表时间：
2000-10-31
影响因子：
1.9
通讯作者：
Gunge, N
中科院分区：
文献类型：
--
作者：
Fukuda, T;Fukuda, K;Gunge, N
利用俄罗斯和平号空间站上的酿酒酵母（Saccharomyces cerevisiae），研究了长期空间飞行对克隆在酵母-大肠杆菌穿梭载体中的细菌核糖体蛋白L基因（rpsL）突变的影响。通过转化E.用从酵母中回收的质粒DNA和rpsL野生型表型（Sm-S）向其突变型表型（Sm-R）的转化的评估，在大肠杆菌中表达。经过40天的太空飞行，部分太空样品的突变频率比地面样品高出2至3倍。核苷酸序列分析表明，空间和地面突变样品之间的点突变率没有明显的差异。然而，大部分的和平号突变体样品被发现有一个总的或大的缺失的rpsL序列，这表明空间辐射含有高线性能量转移（LET）可能会导致缺失型突变。(C)2000 Elsevier Science B. V.保留所有权利。
Using the yeast Saccharomyces cerevisiae on board the Russian space station Mir, we studied the effects of long-term space flight on mutation of the bacterial ribosomal protein L gene (rpsL) cloned in a yeast-Escherichia coli shuttle vector. The mutation frequencies of the cloned rpsL gene on the Mir and the ground (control) yeast samples were estimated by transformation of E. coli with the plasmid DNAs recovered from yeast and by assessment of the conversion of the rpsL wild-type phenotype (Sm-S) to its mutant phenotype (Sm-R). After a 40-day space flight, some part of space samples gave mutation frequencies two to three times higher than those of the ground samples. Nucleotide sequence analysis showed no apparent difference in point mutation rates between the space and the ground mutant samples. However, the greater part of the Mir mutant samples were found to have a total or large deletion in the rpsL sequence, suggesting that space radiation containing high-linear energy transfer (LET) might have caused deletion-type mutations. (C) 2000 Elsevier Science B.V. All rights reserved.