Noninvasive molecular imaging of small living subjects using Raman spectroscopy
Noninvasive molecular imaging of small living subjects using Raman spectroscopy
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DOI：
10.1073/pnas.0710575105
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发表时间：
2008-04-15
影响因子：
11.1
通讯作者：
Gambhir, S. S.
中科院分区：
文献类型：
--
作者：
Keren, S.;Zavaleta, C.;Gambhir, S. S.
随着生物发光和荧光策略的发展，活体分子成像继续迅速发展，特别是经常用于小动物模型。本文介绍了使用拉曼光谱的活体非侵入性深层组织分子图像。我们描述了一种基于拉曼光谱和拉曼纳米粒子的小动物光学成像策略。利用一种适用于小动物拉曼成像的成像系统，利用表面增强拉曼散射纳米颗粒和单壁碳纳米管来演示全身拉曼成像、纳米颗粒药代动力学、多路复用和体内肿瘤靶向。这里报道的成像方式作为活体生物医学成像策略具有重要的潜力。
Molecular imaging of living subjects continues to rapidly evolve with bioluminescence and fluorescence strategies, in particular being frequently used for small-animal models. This article presents noninvasive deep-tissue molecular images in a living subject with the use of Raman spectroscopy. We describe a strategy for small-animal optical imaging based on Raman spectroscopy and Raman nanoparticles. Surf ace-enhanced Raman scattering nanoparticles and single-wall carbon nanotubes were used to demonstrate whole-body Raman imaging, nanoparticle pharmacokinetics, multiplexing, and in vivo tumor targeting, using an imaging system adapted for small-animal Raman imaging. The imaging modality reported here holds significant potential as a strategy for biomedical imaging of living subjects.