Transcriptome analysis shows differential gene expression in the saprotrophic to parasitic transition of Pochonia chlamydosporia.
Transcriptome analysis shows differential gene expression in the saprotrophic to parasitic transition of Pochonia chlamydosporia.
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转录组分析显示厚垣厚孢菌腐养向寄生转变过程中基因表达的差异。
DOI：
10.1007/s00253-011-3282-7
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发表时间：
2011
影响因子：
5
通讯作者：
Rosso LC
中科院分区：
文献类型：
--
作者：
Rosso LC
通过dna扩增片段长度多态性方法，鉴定了植物寄生线虫生物防治剂衣孢pochonia chlamydosporia的表达谱。通过体外实验，对两株不同寄主范围的分离株IMI 380407和IMI 331547在腐生向寄生转变的条件下进行了研究。在三种不同的营养条件和四次采样时间下，用宿主线虫globodera pallidaa和meloidogyne incognita的卵生成基因表达谱。在不同的营养胁迫下（有/没有鸡蛋的饥饿，或丰富的生长介质），获得的RNA指纹中转录本的表达发生了变化。从表达谱中获得的转录本衍生片段（TDFs）对应于6800个产品。对一个子集进行测序，并通过RT - PCR确认其表达谱。根据与翻译或注释序列的相似性，共选择57个tdf进行进一步分析。IMI 380407和IMI 331547在卵寄生过程中表达的基因涉及代谢功能、细胞信号调节、细胞运输、基因表达调节、DNA修复等未知功能。多因素分析显示，IMI 380407和IMI 331547分别有3组和1组TDF表达，均以卵寄生相关基因的表达为特征。两个分离株的TDF集群的共同扩增谱也反映了一个组成基因池，不受营养条件和线虫关联的影响，与一般代谢功能有关。寄生相关基因的差异表达表明，一个诱导/抑制产物网络在真菌信号传导和感染中发挥作用，在宿主感染和寄生性状中有部分重叠。
Expression profiles were identified in the fungusPochonia chlamydosporia, a biological control agent of plant parasitic nematodes, through a cDNA-amplified fragment length polymorphism approach. Two isolates with different host ranges, IMI 380407 and IMI 331547, were assayed in conditions of saprotrophic-to-parasitic transition, through in vitro assays. Gene expression profiles from three different nutritional conditions and four sampling times were generated, with eggs of host nematodesGlobodera pallidaandMeloidogyne incognita. Expression of transcripts changed in RNA fingerprints obtained under different nutritional stresses (starvation in presence/absence of eggs, or rich growth media). Transcript derived fragments (TDFs) obtained from the expression profiles corresponded to 6,800 products. A subset was sequenced and their expression profile confirmed through RT PCR. A total of 57 TDFs were selected for further analysis, based on similarities to translated or annotated sequences. Genes expressed during egg parasitism for both IMI 380407 and IMI 331547 were involved in metabolic functions, cellular signal regulation, cellular transport, regulation of gene expression, DNA repair, and other unknown functions. Multivariate analysis of TDF expression showed three groups for IMI 380407 and one for IMI 331547, each characterized by expression of genes related to eggs parasitism. Common amplification profiles among TDF clusters from both isolates also reflected a pool of constitutive genes, not affected by the nutritional conditions and nematode associations, related to general metabolic functions. The differential expression of parasitism related genes suggest a network of induced/repressed products, playing a role in fungal signaling and infection, with partial overlaps in host infection and parasitism traits.