Functional activities and cellular localization of the ezrin, radixin, moesin (ERM) and RING zinc finger domains in MIR
Functional activities and cellular localization of the ezrin, radixin, moesin (ERM) and RING zinc finger domains in MIR
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DOI：
10.1016/s0014-5793(03)01010-x
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发表时间：
2003-10-09
期刊：
影响因子：
3.5
通讯作者：
Lindholm, D
中科院分区：
文献类型：
--
作者：
Bornhauser, BC;Johansson, C;Lindholm, D
肌球蛋白调节轻链相互作用蛋白（MIR）属于ezrin、radixin、moesin（ERM）蛋白家族，参与膜细胞骨架相互作用和细胞动力学。除了ERM结构域之外，MIR还含有RING锌指区。免疫细胞化学显示全长MIR和亚结构域在细胞中的定位不同。细胞分级分离揭示了类似的分布的全长MIR和环结构域蛋白的Triton X-100不溶性馏分。MIR的轴突生长抑制活性归因于RING结构域。根据完整的RING结构域和蛋白酶体活性控制细胞中的MIR水平。MIR的动态调节有助于其对神经突起生长和细胞运动的影响。(C)2003年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Myosin regulatory light chain interacting protein (MIR) belongs to the ezrin, radixin, moesin (ERM) family of proteins involved in membrane cytoskeleton interactions and cell dynamics. MIR contains, beside the ERM domain, a RING zinc finger region. Immunocytochemistry showed that full-length MIR and the subdomains localize differently in cells. Cell fractionation revealed a similar distribution of full-length MIR and the RING domain protein in the Triton X-100-insoluble fraction. The neurite outgrowth inhibitory activity of MIR was attributed to the RING domain. MIR levels were controlled in the cells depending on the intact RING domain and proteasome activity. The dynamic regulation of MIR contributes to its effects on neurite outgrowth and cell motility. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.