Four functionally distinct C-type natriuretic peptides found in fish reveal evolutionary history of the natriuretic peptide system
Four functionally distinct C-type natriuretic peptides found in fish reveal evolutionary history of the natriuretic peptide system
复制标题
DOI：
10.1073/pnas.1632368100
复制
发表时间：
2003-08-19
影响因子：
11.1
通讯作者：
Takei, Y
中科院分区：
文献类型：
--
作者：
Inoue, K;Naruse, K;Takei, Y
利钠肽(NPs)是脊椎动物体内主要的心血管和渗透压调节激素。虽然四足动物一般有三个亚型，即心房型NP(ANP)、B型NP(BNP)和C型NP(CNP)，但一些硬骨鱼缺乏BNP，而鲨鱼和七鳃鳗只有一种NP。因此，NPs在鱼类进化过程中出现了分化，可能反映了渗透调节系统的变化。在这项研究中，我们通过克隆cnp的方法，在青竹和河豚中发现了四个不同的cnp(1到4)，尽管据我们所知，不超过两个cnp是从脊椎动物物种中分离出来的。预测的成熟CNP-1与哺乳动物CNP最相似，而CNP-4最不相似。然而，人工合成的CNP-4最有效地激活了在培养细胞中表达的青蛙CNP特异性受体(NPR-B)OlGC1，而CNP-1和CNP-3分别最有效地激活了ANP/BNP受体(NPR-A)的两个青竹同源物OlGC7和OlGC2。鱼和人的比较基因组分析将4个CNP基因定位在分别对应于人类1、2、12和17号染色体的青竹染色体上。根据保守的共性，我们发现了以下NPs的进化史：(1)4个CNP基因是在纤支分化之前通过染色体复制产生的；(H)CNP-3基因在四足和硬骨分化之前通过串联复制产生了ANP和BNP基因；(Fit)CNP-1和CNP-2基因在硬骨鱼谱系中保留了，但在四足动物谱系中没有保留；(Iv)CNP-3基因在两栖动物分化后从四足动物谱系中消失；以及(V)CNP-4基因在人类中保留为CNP。
Natriuretic peptides (NPs) are major cardiovascular and osmoregulatory hormones in vertebrates. Although tetrapods generally have three subtypes, atrial NP (ANP), B-type NP (BNP), and C-type NP (CNP), some teleosts lack BNP, and sharks and hagfish have only one NP. Thus, NPs have diverged during fish evolution, possibly reflecting changes in osmoregulatory systems. In this study, we found, by cDNA cloning, four distinct CNPs (1 through 4) in the medaka (Oryzias latipes) and puffer fish (Takifugu rubripes), although to our knowledge no more than two CNPs have been isolated from a vertebrate species. Predicted mature CNP-1 was most similar, and CNP-4 was most dissimilar, to mammalian CNPs. However, synthetic CNP-4 most potently activated OlGC1, a medaka CNP-specific receptor (NPR-B) expressed in cultured cells, whereas CNP-1 and CNP-3 most activated OlGC7 and OlGC2, two medaka homologues of the ANP/BNP receptor (NPR-A), respectively. Linkage mapping in medaka followed by comparative genomic analyses among fishes and humans located four CNP genes in separate medaka chromosomes corresponding to human chromosomes 1, 2, 12, and 17. From conserved synteny, the following evolutionary history of NPs was evoked: (i) four CNP genes were generated by chromosomal duplications before the divergence of elasmobranchs; (h) the CNP-3 gene generated ANP and BNP genes through tandem duplication before the divergence of tetrapods and teleosts; (fit) CNP-1 and -2 genes were retained in the teleost lineage but not in the tetrapod lineage; (iv) the CNP-3 gene disappeared from the tetrapod lineage after divergence of amphibians; and (v) the CNP-4 gene is retained in humans as CNP.