Comparison of in vitro viability methods for Cryptosporidium oocysts
Comparison of in vitro viability methods for Cryptosporidium oocysts
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DOI：
10.1016/j.exppara.2018.03.002
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发表时间：
2018-04-01
影响因子：
2.1
通讯作者：
Zintl, Annetta
中科院分区：
文献类型：
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作者：
Vande Burgt, Nathan H.;Auer, Agathe;Zintl, Annetta
水生原生动物寄生虫隐孢子虫形成的卵囊可以在环境中持续很长一段时间，即使卵囊内的子孢子可能不再存活，这使得难以评估相关的感染风险。在这项研究中，我们比较了各种体外方法区分活卵囊和非活卵囊的能力，包括脱囊、DAPI/PI染色、RNA FISH、PMA-qPCR和一种新的聚合物载玻片粘附方法。除了我们的体外脱囊方案之外，发现所有方法都可用于鉴定活卵囊。(C)2018爱思唯尔公司All rights reserved.
The water-borne protozoan parasite Cryptosporidium parvum forms oocysts that can persist for long periods of time in the environment, even though the sporozoites inside the oocysts may no longer be viable, making it difficult to assess the associated risk of infection. In this study, we compared the ability of various in vitro methods to discriminate viable from non-viable oocysts, including excystation, DAPI/PI staining, RNA FISH, PMA-qPCR and a novel polymer slide adhesion method. With the notable exception of our in vitro excystation protocol, all methods were found to be useful for identifying viable oocysts. (C) 2018 Elsevier Inc. All rights reserved.