Inverse correlation between RASSF1A hypermethylation, KRAS and BRAF mutations in cervical adenocarcinoma
Inverse correlation between RASSF1A hypermethylation, KRAS and BRAF mutations in cervical adenocarcinoma
复制标题
DOI：
10.1016/j.ygyno.2007.01.045
复制
发表时间：
2007-06-01
影响因子：
4.7
通讯作者：
Dong, Seung Myung
中科院分区：
文献类型：
--
作者：
Kang, Sokbom;Kim, Hy-Sook;Dong, Seung Myung
客观的。尽管宫颈腺癌的发病率不断增加，但其进展过程中的遗传和表观遗传变化却很少被描述。我们推测RASSF1A甲基化以及KRAS和BRAF突变可能在宫颈腺癌中发挥重要作用。方法。使用甲基化特异性 PCR 和特异性序列分析，评估了由宫颈腺癌 (17 = 115) 和鳞状细胞癌 (n = 143) 组成的宫颈原发癌组织 (n = 258) 的 BRAF 和 KRAS 激活突变以及 RASSF1A 启动子高甲基化。 HPV E7 型特异性 PCR 用于检测 HPV-16 和 -18 状态。结果。在 16 例腺癌（13.9%）中发现了 KRAS 突变，在 5 例（4.3%）中发现了 BRAF 突变。 RASSF1A 甲基化存在于 27 种腺癌 (23.5%) 中，并且与宫颈腺癌中的 KRAS 和/或 BRAF 突变呈负相关 (p = 0.002)。在宫颈鳞状细胞癌中，仅在 1 例（0.7%）病例中检测到 KRAS 突变，在 2 例（1.4%）例中检测到 RASSF1A 高甲基化。 KRAS 突变和 RASSF1A 甲基化的频率在腺癌之间存在显着差异（P
Objective. Although the incidence of cervical adenocarcinoma is increasing, few genetic and epigenetic changes in its progression have been described. We hypothesized that RASSF1A methylation and KRAS and BRAF mutations may play an important role in cervical adenocarcinoma.Methods. Archival primary carcinoma tissues (n = 258) in uterine cervix consisting cervical adenocarcinomas (17 = 115) and squamous cell carcinomas (n = 143) were evaluated for activating mutations of BRAF and KRAS and promoter hypermethylation of RASSF1A using methylation specific PCR and specific sequence analysis. HPV E7 Type-specific PCR was used for HPV-16 and -18 status.Results. KRAS mutations were found in 16 adenocarcinomas (13.9%), while BRAF mutations were found in 5 (4.3%). RASSF1A methylation was found in 27 adenocarcinomas (23.5%) and inversely correlated with KRAS and/or BRAF mutation (p = 0.002) in cervical adenocarcinoma. In cervical squamous cell carcinomas, KRAS mutations were detected only in 1 (0.7%) cases and RASSF1A hypermethylation was detected in 2 (1.4%). The frequency of KRAS mutation and RASSF1A methylation were significantly different between adenocarcinomas (P