Isolation and characterization of the Cryptococcus neoformans MATa pheromone gene.
Isolation and characterization of the Cryptococcus neoformans MATa pheromone gene.
复制标题
新型隐球菌 MATa 信息素基因的分离和表征。
DOI：
10.1093/genetics/160.3.935
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发表时间：
2002
期刊：
影响因子：
3.3
通讯作者：
Wickes,BrianL
中科院分区：
文献类型：
--
作者：
McClelland,CarolM;Fu,Jianmin;Woodlee,GayL;Seymour,TaraS;Wickes,BrianL
新型隐球菌是一种异宗配合的担子菌，有两种交配型，MAT α和MAT Ta.该真菌的交配途径中有许多保守基因，包括aMATα特异性信息素（MFα1）。采用改良的差异显示策略鉴定了一个编码MAT信息素的基因。该基因命名为MFa1，全长42个氨基酸，含有一个保守的法尼基化基序，以三个连锁拷贝存在，跨越一个20 kb的MAT特异性DNA片段，并定位于含MAT的染色体上。转化研究表明MFa1仅在MAT α细胞中诱导纤维形成，表明MFa1在功能上是保守的。对预测的Mfa1和Mfα1蛋白的序列分析表明，与其他真菌如酿酒酵母相比，C. neoformanspheromone基因在结构和功能上是保守的。然而，与在两种C. MFa1对C.新人类
Cryptococcus neoformansis a heterothallic basidiomycete with two mating types,MATaandMATα. The mating pathway of this fungus has a number of conserved genes, including aMATα-specific pheromone (MFα1). A modified differential display strategy was used to identify a gene encoding theMATapheromone. The gene, designatedMFa1, is 42 amino acids in length and contains a conserved farnesylation motif.MFa1is present in three linked copies that span a 20-kb fragment ofMATa-specific DNA and maps to theMAT-containing chromosome. Transformation studies showed thatMFa1induced filament formation only inMATα cells, demonstrating thatMFa1is functionally conserved. Sequence analysis of the predicted Mfa1 and Mfα1 proteins revealed that, in contrast to other fungi such asSaccharomyces cerevisiae, theC. neoformanspheromone genes are structurally and functionally conserved. However, unlike theMFα1gene, which is found inMATα strains of both varieties ofC. neoformans, MFa1is specific for theneoformansvariety ofC. neoformans.