A Multi-Functional Microelectrode Array Featuring 59760 Electrodes, 2048 Electrophysiology Channels, Stimulation, Impedance Measurement and Neurotransmitter Detection Channels.
A Multi-Functional Microelectrode Array Featuring 59760 Electrodes, 2048 Electrophysiology Channels, Stimulation, Impedance Measurement and Neurotransmitter Detection Channels.
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DOI：
10.1109/jssc.2017.2686580
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发表时间：
2017-06
影响因子：
5.4
通讯作者：
Hierlemann A
中科院分区：
文献类型：
--
作者：
Dragas J;Viswam V;Shadmani A;Chen Y;Bounik R;Stettler A;Radivojevic M;Geissler S;Obien M;Müller J;Hierlemann A
生物细胞的特征是高度复杂的现象和过程，这些现象和过程在很大程度上相互依赖。为了获得详细的见解，设计用于研究细胞现象的设备需要能够并行跟踪和操纵多个细胞参数;它们必须提供高信号质量和高时空分辨率。为此，我们开发了一种基于CMOS的微电极阵列系统，集成了六种测量和刺激功能，这是迄今为止数量最多的。此外，该系统具有迄今为止最大的有源电极阵列面积（4.48×2.43 mm 2），可容纳59，760个电极，而其功耗、噪声特性和空间分辨率（13.5 μm电极间距）与最先进的设备相当。该系统包括：2，048个动作电位（AP，带宽：300 Hz至10 kHz）记录单元、32个局部场电位（LFP，带宽：1 Hz至300 Hz）记录单元、32个电流记录单元、32个阻抗测量单元和28个神经递质检测单元，以及16个双模式电压或电流/电压控制刺激单元。电极阵列架构基于开关矩阵，其允许将任何测量/刺激单元连接到阵列中的任何电极，并且允许并行执行不同的测量/刺激功能。
Biological cells are characterized by highly complex phenomena and processes that are, to a great extent, interdependent. To gain detailed insights, devices designed to study cellular phenomena need to enable tracking and manipulation of multiple cell parameters in parallel; they have to provide high signal quality and high spatiotemporal resolution. To this end, we have developed a CMOS-based microelectrode array system that integrates six measurement and stimulation functions, the largest number to date. Moreover, the system features the largest active electrode array area to date (4.48×2.43 mm2) to accommodate 59,760 electrodes, while its power consumption, noise characteristics, and spatial resolution (13.5 μm electrode pitch) are comparable to the best state-of-the-art devices. The system includes: 2,048 action-potential (AP, bandwidth: 300 Hz to 10 kHz) recording units, 32 local-field-potential (LFP, bandwidth: 1 Hz to 300 Hz) recording units, 32 current recording units, 32 impedance measurement units, and 28 neurotransmitter detection units, in addition to the 16 dual-mode voltage-only or current/voltage-controlled stimulation units. The electrode array architecture is based on a switch matrix, which allows for connecting any measurement/stimulation unit to any electrode in the array and for performing different measurement/stimulation functions in parallel.