Immunophenotyping analysis of peripheral blood, splenic, and thymic lymphocytes in male and female rats
Immunophenotyping analysis of peripheral blood, splenic, and thymic lymphocytes in male and female rats
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DOI：
10.1016/s1056-8719(96)00146-3
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发表时间：
1997-02-01
影响因子：
1.9
通讯作者：
Komocsar, WJ
中科院分区：
文献类型：
--
作者：
Morris, DL;Komocsar, WJ
使用流式细胞术方法来表征 Sprague-Dawley 大鼠中淋巴组织相关的淋巴细胞亚群。来自雄性和雌性大鼠的外周血、脾脏和胸腺的单核白细胞用一组针对特定细胞表面蛋白的荧光标记单克隆和多克隆抗体进行标记。当通过流式细胞术进行分析时，这些标记蛋白的差异表达用于在表型上区分淋巴细胞的一个亚群与另一个亚群。该方法用于确定每种淋巴组织中总 B 细胞（CD45RA+；sIgM+）、总 T 细胞（CD3+；pan-T+）、辅助性 T 细胞（CD3+CD4+）、细胞毒性/抑制性 T 细胞（CD3+CD8+）以及 CD4:CD8 比率的相对百分比和绝对数量。此外，使用双参数分析区分胸腺中所有分化 T 细胞亚群（即 CD4+CD8-、CD4-CD8+、CD4+CD8+ 和 CD4-CD8- 细胞）。本研究的结果表明：1) 本研究中使用的选定抗体组可以识别大鼠血液、脾脏和胸腺中存在的所有淋巴细胞亚群，2) 雄性和雌性 Sprague-Dawley 大鼠在选定的淋巴组织中存在的一些淋巴细胞亚群的比例显示出轻微但不具有统计学意义的差异。这种流式细胞术方法可用于准确评估异生物质的潜在免疫毒性或免疫调节作用，其特征是大鼠表型表达模式的变化或淋巴细胞亚群数量的变化。 (C) 1997 爱思唯尔科学公司。
A flow cytometry method was used to characterize lymphoid tissue-associated lymphocyte subsets in the Sprague-Dawley rat. Mononuclear leukocytes from peripheral blood, spleen, and thymus of male and female rats were labeled with a panel of fluorescently tagged monoclonal and polyclonal antibodies directed against specific cell-surface proteins. The differential expression of these marker proteins was used to phenotypically distinguish one subpopulation of lymphocytes from another when analyzed via flow cytometry. This method was used to determine the relative percentages and absolute number of total B cells (CD45RA+; sIgM+), total T cells (CD3+; pan-T+), helper T cells (CD3+CD4+), cytotoxic/suppressor T cells (CD3+CD8+), and the CD4:CD8 ratio in each of these lymphoid tissues. Additionally, all subsets of differentiating T cells in the thymus (i.e., CD4+CD8-, CD4-CD8+, CD4+CD8+, and CD4-CD8- cells) were distinguished using dual parameter analyses. Results of this study demonstrate that 1) the selected panel of antibodies used in this study can identify all lymphocyte subsets present in blood, spleen, and thymus of the rat and 2) male and female Sprague-Dawley rats show slight, but not statistically significant, differences in the proportions of some lymphocyte subsets present in select lymphoid tissues. This flow cytometry method can be used to accurately assess the potential immunotoxic or immunomodulatory effect of xenobiotic agents as characterized by changes in the phenotypic expression patterns or alterations in the quantity of lymphocyte subpopulations in the rat. (C) 1997 Elsevier Science Inc.