CROSS CONTAMINATION ASSOCIATED WITH THE USE OF MULTIWELL CULTURE PLATES FOR CYTOTOXICITY ASSESSMENT OF VOLATILE CHEMICALS
CROSS CONTAMINATION ASSOCIATED WITH THE USE OF MULTIWELL CULTURE PLATES FOR CYTOTOXICITY ASSESSMENT OF VOLATILE CHEMICALS
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DOI：
10.1007/bf00353705
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发表时间：
1991-05-01
期刊：
影响因子：
2.2
通讯作者：
ADOLPHE, M
中科院分区：
文献类型：
--
作者：
BLEIN, O;RONOT, X;ADOLPHE, M
由于挥发性测试化学品的蒸发，体外毒性测试可能会涉及技术问题。 两种挥发性化学物质（丁醇和乙醇）的细胞毒性已在传统微量滴定板中通过中性红测定进行了评估。 非挥发性 DMSO 化学品用作阴性对照。 在这些条件下，观察到测试浓度组之间存在重要的交叉污染。 这会影响细胞毒性估计，导致细胞毒性估计被高估。 当使用含有可拆卸杆的特殊板时，可以防止这种交叉污染。
In vitro toxicity testing can involve technical problems due to the evaporation of volatile test chemicals. The cytotoxicity of two volatile chemicals (butanol and ethanol) has been assessed with neutral red assay in conventional microtiter plates. The non volatile DMSO chemical is used as a negative control. Under these conditions, an important cross contamination between test concentration groups has been observed. This affects cytotoxicity estimation which is overestimated. This cross contamination is prevented when special plates containing removable bars are used.