Microscale patchiness of bacterioplankton assemblage richness in seawater
Microscale patchiness of bacterioplankton assemblage richness in seawater
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DOI：
10.3354/ame026103
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发表时间：
2001-12-05
影响因子：
1.4
通讯作者：
Azam, F
中科院分区：
文献类型：
--
作者：
Long, RA;Azam, F
我们试图测试的假设，细菌物种丰富度和组成变化在毫米尺度的海洋浮游环境中，响应于有机物的异质性分布。为了检验这一假设，有必要设计和测试一个协议，样品，裂解和分析（聚合酶链反应/变性梯度凝胶电泳[PCR-DGGE]）浮游细菌物种丰富度在一微升的海水。在1穆尔样品中发现细菌物种丰富度的显着变化，更大的物种丰富度被认为是当海水中富含硅藻碎屑，在20 mm的样带上以2 mm的间隔检查的物种丰富度显示出更大的变异性时，在峰丰度的Lingulodinium polyedrum相比，晚期的盛开。在富含有机颗粒的样品中，总体物种丰富度最高。这些结果表明，颗粒有机物可能，在一定程度上，调节细菌物种丰富度的水平，以及造成斑块的小尺度分布的物种，微尺度斑块的细菌物种可能有重要的意义，不仅在微尺度，而且在海洋盆地尺度的碳循环和生态地球化学。
We sought to test the hypothesis that bacterial species richness and composition vary at the millimeter scale in the marine pelagic environment, in response to the heterogeneous distribution of organic matter. To test this hypothesis, it was necessary to design and test a protocol to sample, lyse and analyze (by polymerase chain reaction/denaturing gradient gel electrophoresis [PCR-DGGE]) bacterioplankton species richness in a single microliter of seawater. Significant variation in bacterial species richness was found amongst 1 mul samples, Greater species richness was seen when seawater was enriched with diatom detritus, Examination of species richness at 2 mm intervals over a 20 mm transect showed greater variability during the peak abundance of Lingulodinium polyedrum when compared to the late stage of the bloom. Overall species richness was highest in samples enriched with organic particles. These results suggest that particulate organic matter may, in part, regulate the level of bacterial species richness as well as cause patchiness in the small-scale distribution of species, Microscale patchiness of bacterial species may have important implications in carbon cycling and biogeochemistry, not only at the microscale but also at the ocean basin scale.