Low-density lipoprotein receptor affects the fertility of female mice
Low-density lipoprotein receptor affects the fertility of female mice
复制标题
低密度脂蛋白受体影响雌性小鼠的生育能力。
DOI：
10.1071/rd13436
复制
发表时间：
2015-01-01
影响因子：
1.9
通讯作者：
Zhang, Cong
中科院分区：
文献类型：
--
作者：
Guo, Tao;Zhang, Liang;Zhang, Cong
低密度脂蛋白受体（LDLR）已被证明在脂蛋白代谢中发挥核心作用，LDLR缺陷（LDLR(-/-)）小鼠发生严重的血脂异常。在本研究中，我们研究了Ldlr基因敲除是否会损害雌性生殖，并探讨了其中的机制。结果表明，虽然Ldlr（-/-）小鼠的产仔数与对照组的产仔数没有显著差异，但前者的产仔数明显低于对照组。有趣的是，尽管Ldlr（-/-）小鼠肥胖，但它们卵巢的重量比对照组小鼠低。Ldlr（-/-）小鼠的血清胆固醇水平明显高于野生型小鼠。相反，Ldlr（-/-）小鼠卵巢中胆固醇、甘油三酯和总脂质水平显著降低。通过油红O染色检测到的卵巢脂质沉积和透射电镜观察到的脂滴都支持Ldlr（-/-）小鼠卵巢脂质水平降低。此外，Ldlr（-/-）小鼠的卵泡更少，卵泡闭锁更多，雌激素水平更低，发情时间明显少于对照组。超排卵试验表明，未成熟的Ldlr（-/-）小鼠比对照组排卵更少。这些结果表明，缺乏Ldlr会导致血脂异常和生育能力低下。
Low-density lipoprotein receptor (LDLR) has been demonstrated to play a central role in lipoprotein metabolism, with Ldlr-deficient (Ldlr(-/-)) mice developing severe dyslipidemia. In the present study we investigated whether Ldlr knockout could harm female reproduction and explored the mechanisms involved. The results indicate that although the number of litters born to Ldlr(-/-) mice did not differ significantly from that born to controls, the number of pups per litter was significantly lower in the former group. Interestingly, although Ldlr(-/-) mice were obese, the weight of their ovaries was lower than that in control mice. Serum cholesterol levels was significantly higher in Ldlr(-/-) mice than in their wild-type counterparts. In contrast, there were significant decreases in cholesterol, triglyceride and total lipid levels in ovaries of Ldlr(-/-) mice. Both ovarian lipid deposition, as detected by Oil red O staining, and lipid droplets, as evaluated by transmission electron microscopy, supported decreased lipid levels in ovaries from Ldlr(-/-) mice. In addition, Ldlr(-/-) mice had fewer ovarian follicles, more atretic follicles, lower oestrogen levels and spent significantly less time in oestrus than did the controls. Superovulation assays indicated immature Ldlr(-/-) mice ovulated fewer ova than controls. These results indicate that lack of Ldlr results in dyslipidaemia and poor fertility.