A cholinoceptive desynchronized sleep induction zone in the anterodorsal pontine tegmentum: locus of the sensitive region.
A cholinoceptive desynchronized sleep induction zone in the anterodorsal pontine tegmentum: locus of the sensitive region.
复制标题
脑桥被盖前背侧的胆碱感受不同步睡眠诱导区：敏感区域的位置。
DOI：
10.1016/0306-4522(90)90267-8
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发表时间：
1990
期刊：
影响因子：
3.3
通讯作者：
Hobson,JA
中科院分区：
文献类型：
--
作者：
Yamamoto,K;Mamelak,AN;Quattrochi,JJ;Hobson,JA
将长效胆碱能激动剂卡巴胆碱(4μg/250nL/90 S)微量注射到4只猫的脑桥前被盖内90个部位，计算起病时间和40min后脱同步化睡眠状态的时间百分比。与更多的桥脑后腹侧部位相比，观察到的更短的潜伏期和更高的百分比证实了早期关于脑桥前背侧存在敏感胆碱感受区的预测。在27个试验中，在5分钟内观察到不同步的睡眠状态；在31个试验中，潜伏期为5-10分钟，在其余32个试验中，潜伏期大于10分钟。将去同步化睡眠样状态潜伏期和去同步化睡眠样状态百分比作为三维坐标的函数图显示，潜伏期短(<；5min)和高百分比(>；80%)的注射部位集中在P1.0~3.5和V−3.5~−5.5的坐标之间，在L 2.0的横坐标上。在额叶平面上，短的脱同步化睡眠样状态潜伏期和高脱同步化睡眠样状态百分率的部位开始于脑桥被盖区腹侧被盖核，并向腹尾侧延伸3 mm。矢状面数据的回归图显示短潜伏期轴线，短潜伏期部位围绕该轴线呈轻微的背腹方向运行，从P1.0到V−4.0，再到P4.0到V−5.5。这一观察表明，敏感区的形状可能类似于圆柱体，这一假说得到了潜伏期较长和与轴的径向距离增加时百分比较低的相关性的支持。胆碱能潜能与短潜伏期轴距离之间的非线性关系表明，去同步化的睡眠样状态潜伏期是两个因素的函数：基于可变扩散的卡巴胆碱到参与去同步化的类睡眠状态产生的远端神经元群体的延迟，以及在敏感区域神经元激活后基于固定招募的延迟。根据早期涉及脑桥被盖微刺激的研究解释这些发现，支持将离散神经元群体的分布式网络作为去同步化睡眠产生的来源。
Carbachol, a long-acting cholinergic agonist, was microinjected (4 μg/250 nl per 90 s) into 90 sites within the anterodorsal pontine tegmentum of four cats and the time to onset and percentage of time spent in a desynchronized sleep-like state during 40 min postinjection were calculated. Compared with more posteroventral pontine sites, the shorter latencies and higher percentages observed confirmed earlier predictions of a sensitive cholinoceptive zone in the anterodorsal pons. In 27 trials a desynchronized sleep-like state was observed within 5 min; in 31 trials the latency was 5–10 min and in the remaining 32 trials, greater than 10 min. Plotting the desynchronized sleep-like state latency and the desynchronized sleep-like state percentage as a function of the three-dimensional coordinates revealed that injection sites with short latency (<5min) and high percentage (>80%) were concentrated between the coordinates of P 1.0 to 3.5 and V −3.5 to −5.5, at the lateral coordinate L 2.0. On the frontal plane, the short desynchronized sleep-like state latency and high desynchronized sleep-like state percentage sites begin in the pontine tegmental region just lateral to the ventral tegmental nucleus and extend 3 mm ventrocaudally. A regression plot of the data in sagittal plane 2.0 revealed a short latency axis, around which the short latency sites cluster, running in a slightly dorsoventral direction from about P 1.0 to V −4.0 to P 4.0 to V −5.5. This observation suggests that the sensitive zone might approximate a cylinder in shape, a hypothesis supported by the correlation of longer latencies and lower percentages at increasing radial distance from the axis. The non-linear relationship between cholinergic potency and distance from the short latency axis suggests that the desynchronized sleep-like state latency is a function of two factors: a variable diffusion-based delay ofcarbachol to distant neuronal populations involved in the desynchronized sleep-like state production, and a fixed recruitment-based delay following activation of neurons in the sensitive zone.Interpretation of these findings in light of earlier studies involving microstimulation of the pontine tegmentum argue in favor of a distributed network of discrete neuronal populations as the source of desynchronized sleep generation.