Escherichia coli ST131-H22 as a Foodborne Uropathogen.
Escherichia coli ST131-H22 as a Foodborne Uropathogen.
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DOI：
10.1128/mbio.00470-18
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发表时间：
2018-08-28
期刊：
影响因子：
6.4
通讯作者：
Price LB
中科院分区：
文献类型：
--
作者：
Liu CM;Stegger M;Aziz M;Johnson TJ;Waits K;Nordstrom L;Gauld L;Weaver B;Rolland D;Statham S;Horwinski J;Sariya S;Davis GS;Sokurenko E;Keim P;Johnson JR;Price LB
大肠杆菌ST131是一种重要的肠外病原菌，可定植于人和食用动物的胃肠道。在这里，我们将与鸟类适应相关的附属性状(COLV质粒)的检测与高分辨率系统发育学相结合，以量化由食用动物来源的ST131菌株引起的人类感染的部分。我们的结果表明，一个ST131亚种--ST131-H22--已经在世界各地的家禽种群中确立，肉类可能是人类暴露和感染的媒介。ST131-H22只是许多可能从食用动物传播给人类的大肠杆菌谱系之一。将宿主相关辅助元件的检测与系统发育学相结合的其他研究可能使我们能够量化可归因于食用动物大肠杆菌菌株的人类肠外感染的总比例。大肠杆菌序列类型131(ST131)已迅速崛起，成为当今流通中最流行的肠外致病性大肠杆菌克隆。以前的研究似乎证明零售肉类是人类接触ST131的一个来源；然而，这些研究主要集中在广泛耐多药的ST131菌株，它们通常携带fimH 1型菌毛粘附素基因(ST131-H30)的等位基因30。为了在不偏向特定亚型或表型的情况下估计食源性ST131菌株引起的人类肠外感染的频率，我们在亚利桑那州弗拉格斯塔夫对肉制品和临床培养中的大肠杆菌进行了一项为期一年的前瞻性研究。我们通过多位点序列分型、fimH分型和核心基因组系统发育分析对所有分离株进行了鉴定，并筛选了与禽类相关的COLV质粒作为家禽适应的指标。从2452份肉类样本中分离出79.8%的大肠杆菌，从1735份培养阳性的临床样本中分离出72.4%的大肠杆菌。有27株肉类分离株为ST131，其中25株几乎完全属于ST131-H22谱系。25株H22肉类分离株中，除1株来自禽类产品外，其余均携带禽类相关的COLV质粒。在1,188株人类临床分离的大肠杆菌中，有24株是ST131-H22，其中四分之一与ST131-H22肉类分离株存在相同的高分辨率系统发育分支，并携带COLV质粒。对一个国际ST131-H22基因组收集的分子时钟分析表明，自20世纪40年代以来，至少已经获得了六次COLV质粒，家禽传人并不局限于美国。
E. coli ST131 is an important extraintestinal pathogen that can colonize the gastrointestinal tracts of humans and food animals. Here, we combined detection of accessory traits associated with avian adaptation (ColV plasmids) with high-resolution phylogenetics to quantify the portion of human infections caused by ST131 strains of food animal origin. Our results suggest that one ST131 sublineage—ST131-H22—has become established in poultry populations around the world and that meat may serve as a vehicle for human exposure and infection. ST131-H22 is just one of many E. coli lineages that may be transmitted from food animals to humans. Additional studies that combine detection of host-associated accessory elements with phylogenetics may allow us to quantify the total fraction of human extraintestinal infections attributable to food animal E. coli strains. Escherichia coli sequence type 131 (ST131) has emerged rapidly to become the most prevalent extraintestinal pathogenic E. coli clones in circulation today. Previous investigations appeared to exonerate retail meat as a source of human exposure to ST131; however, these studies focused mainly on extensively multidrug-resistant ST131 strains, which typically carry allele 30 of the fimH type 1 fimbrial adhesin gene (ST131-H30). To estimate the frequency of extraintestinal human infections arising from foodborne ST131 strains without bias toward particular sublineages or phenotypes, we conducted a 1-year prospective study of E. coli from meat products and clinical cultures in Flagstaff, Arizona. We characterized all isolates by multilocus sequence typing, fimH typing, and core genome phylogenetic analyses, and we screened isolates for avian-associated ColV plasmids as an indication of poultry adaptation. E. coli was isolated from 79.8% of the 2,452 meat samples and 72.4% of the 1,735 culture-positive clinical samples. Twenty-seven meat isolates were ST131 and belonged almost exclusively (n = 25) to the ST131-H22 lineage. All but 1 of the 25 H22 meat isolates were from poultry products, and all but 2 carried poultry-associated ColV plasmids. Of the 1,188 contemporaneous human clinical E. coli isolates, 24 were ST131-H22, one-quarter of which occurred in the same high-resolution phylogenetic clades as the ST131-H22 meat isolates and carried ColV plasmids. Molecular clock analysis of an international ST131-H22 genome collection suggested that ColV plasmids have been acquired at least six times since the 1940s and that poultry-to-human transmission is not limited to the United States.