New cineromycins and musacins obtained by metabolite pattern analysis of Streptomyces griseoviridis (FH-S 1832) .1. Taxonomy, fermentation, isolation and biological activity
New cineromycins and musacins obtained by metabolite pattern analysis of Streptomyces griseoviridis (FH-S 1832) .1. Taxonomy, fermentation, isolation and biological activity
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DOI：
10.7164/antibiotics.49.432
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发表时间：
1996-05-01
影响因子：
3.3
通讯作者：
Zeeck, A
中科院分区：
文献类型：
--
作者：
Burkhardt, K;Fiedler, HP;Zeeck, A
使用薄层色谱法和各种染色试剂进行化学筛选提供了一个机会，以可视化的微生物次级代谢物模式（代谢指纹）的几乎完整的图片。这种方法可以有利地用于检测所谓的“天才”菌株和鉴定微生物菌株集合，特别是作为有效应用的生物高通量测定的基本步骤。根据其代谢指纹，微生物分离株可分为：（i）非生产性生物体，其不显示次级代谢物的形成-达到规定的检测限，（ii）生产性狭窄的生物体，其产生一种或两种次级代谢物作为主要产物，对培养条件的改变具有有限的依赖性，和（iii）天赋生物体，它们能够合成一系列结构不同的次级代谢物。以灰绿链霉菌FH-S1832为例，对该菌株进行了详细的研究。报道了其分类学特征、发酵以及分离和纯化程序的研究，这些程序导致了14元大环内酯的cineromycin型（cineromycin B和三个新的同系物）和musacins A至F。Musacin C具有抗蠕虫和弱的抗病毒活性。
Chemical screening using thin layer chromatography and various staining reagents offers the opportunity to visualize a nearly complete picture of a microbial secondary metabolite pattern (metabolic finger-print). This approach can be used advantageously for both, the detection of so-called ''talented'' strains, and for qualifying microbial strain collections, especially as a fundamental step of efficiently applied biological high-throughput assays. Based on their metabolic finger-print, microbial isolates can be classified in: (i) non-producing organisms, which gave no indication of the formation of secondary metabolites-up to a defined detection limit, (ii) organisms of narrow productivity, which produce one or two secondary metabolites as main products with a restricted dependance to alteration of the culture conditions, and (iii) talented organisms, which are able to synthesize an array of structurally different secondary metabolites. As an example, the talented strain, Streptomyces griseoviridis (FH-S 1832), was studied in detail. Investigations in its taxonomical characterization, fermentation, as well as the isolation and purification procedures leading to 14-membered macrocyclic lactones of the cineromycin-type (cineromycin B and three new congeners) and to the musacins A to F are reported. Musacin C exhibits anthelminthic and weak antiviral activities.