DNA methylation as a mechanism of nutritional plasticity: limited support from horned beetles.
DNA methylation as a mechanism of nutritional plasticity: limited support from horned beetles.
复制标题
DNA 甲基化作为营养可塑性的机制：来自角甲虫的有限支持。
DOI：
10.1002/jez.b.22479
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Moczek,ArminP
中科院分区：
文献类型：
--
作者：
Snell-Rood,EmilieC;Troth,Ashley;Moczek,ArminP
DNA的表观遗传变化，即序列水平上的潜在遗传改变，如DNA甲基化，被认为是许多适应性表型可塑性的基础。我们对自然群体中表观遗传变异和适应性表型可塑性之间联系的理解是有限的。如果DNA甲基化是对不同营养环境的适应性反应的基础，那么甲基化模式应该与营养环境中的表现差异相关，并对环境的变化做出反应。此外，可以应对更广泛营养环境的基因型预计在甲基化模式方面具有更大的灵活性。我们测试了这些预测使用角甲虫（属Onthophagus），它可以应付广泛的变化，幼虫营养。我们使用甲基化特异性扩增片段长度多态性（AFLP）分析调查了来自自然种群的实验室饲养的甲虫中几个甲基化位点的甲基化水平。对于不到一半的研究位点，甲基化水平与性能相关，在不同的营养环境中，在给定的饮食中获得成年人的身体大小，男性（更具营养可塑性的性别）比女性的整体效果更大。在大多数网站的甲基化水平的影响更多的基因型（等雌性线）比环境（粪便类型）。只有1个位点（共12个）显示出显著的基因型与环境相互作用。总之，我们的研究结果为DNA甲基化是营养可塑性基础的假设提供了适度的支持，因为只有8 - 16%的甲基化位点符合我们的所有预测。J. Exp. Zool.（Mol. dev. Evol.）320 B：22 - 34，2013.© 2012 Wiley Periodicals，Inc.
Epigenetic changes to DNA, potentially heritable alterations above the sequence level, such as DNA methylation, are thought to underlie many instances of adaptive phenotypic plasticity. Our understanding of the links between epigenetic variation and adaptive phenotypic plasticity in natural populations is limited. If DNA methylation underlies adaptive responses to different nutritional environments, methylation patterns should be correlated with differences in performance across nutritional environments, and respond to changes in the environment. Additionally, genotypes that can cope with a broader range of nutritional environments are expected to have greater flexibility in methylation patterns. We tested these predictions using horned beetles (genusOnthophagus), which can cope with a wide range of variation in larval nutrition. We surveyed levels of methylation across several methylated loci in lab‐reared beetles originating from natural populations using a methylation‐specific amplified fragment length polymorphism (AFLP) analysis. For less than half the of the loci investigated, methylation level was correlated with performance, measured as adult body size attained on a given diet, in different nutritional environments, with an overall greater effect in males (the more nutritionally plastic sex) than females. Methylation levels at most sites were influenced more by genotype (iso‐female line) than by environment (dung type). Only 1 site (of 12) showed a significant genotype‐by‐environment interaction. Taken together, our results provide modest support for the hypothesis that DNA methylation underlies nutritional plasticity, as only 8–16% of methylated sites conformed to all of our predictions. J. Exp. Zool. (Mol. Dev. Evol.) 320B:22–34, 2013. © 2012 Wiley Periodicals, Inc.
登录
查看更多内容
DOI：
10.2307/2419653
发表时间：
1998-05
期刊：
--
影响因子：
--
作者：
C. Schlichting;M. Pigliucci
通讯作者：
C. Schlichting;M. Pigliucci
影响因子：
3.5
作者：
Sandovici, I;Leppert, M;Sapienza, C
通讯作者：
Sapienza, C
影响因子：
64.8
作者：
通讯作者：
--
DOI：
10.1098/rspb.1997.0081
发表时间：
1997-04-22
影响因子：
4.7
作者：
Emlen, DJ
通讯作者：
Emlen, DJ
影响因子：
2
作者：
Lachmann, M;Jablonka, E
通讯作者：
Jablonka, E