Microperimetry as an Outcome Measure in Choroideremia Trials: Reproducibility and Beyond
Microperimetry as an Outcome Measure in Choroideremia Trials: Reproducibility and Beyond
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DOI：
10.1167/iovs.16-19338
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发表时间：
2016-08-01
影响因子：
4.4
通讯作者：
MacDonald, Ian M.
中科院分区：
文献类型：
--
作者：
Dimopoulos, Ioannis S.;Tseng, Calvin;MacDonald, Ian M.
目的.采用标准刺激网格和个性化刺激网格对无脉络膜（CHM）受试者进行微视野检查（MP），以确定其重测重复性。15名CHM受试者（28只眼）使用标准（108）和适应个体黄斑病理的定制黄斑网格进行黄斑完整性评估（马亚）微视野连续重复检查。标准网格平均值（MS）和逐点（PWS）的灵敏度的重复性进行了测定，并与年龄匹配的对照组（7只眼）进行了比较，PWS分别分析了变性边界内外的位点。插值体积指数被用来估计定制网格的可重复性，并将其性能与标准网格进行比较。标准网格MS的重测结果显示，CHM受试者的变异系数（CV）高于对照组（0.09 vs. 0.02）。自定义网格的体积指数通过将CV值驱动到0.05和接近0.02来提高感兴趣区域（ROI）分析的可重复性。CHM组PWS变异性显著高于对照组，尤其是在视网膜中央变性边缘（10.68dB vs.4.74dB，P < 0.001）。CHM的微视野检查在变性边缘显示出较高的重测变异，这影响了MS测量的重复性。定制网格的体积测量可以提高全球和区域敏感性评估的可靠性。然而，在评估潜在的功能下降和/或疾病进展时，需要考虑单个点的固有重测变异。
PURPOSE. To determine test-retest repeatability of microperimetry testing (MP) in choroideremia (CHM) subjects using standard and personalized stimulus grids.METHODS. Fifteen CHM subjects (28 eyes) underwent consecutive repeat examinations with the Macular Integrity Assessment (MAIA) microperimeter using a standard (108) and a customized macular grid adapted to individual macular pathology. Repeatability of standard-grid mean (MS) and point-wise (PWS) sensitivity was determined and compared with age-matched controls (seven eyes), with PWS separately analyzed for loci within and outside the border of degeneration. Interpolated volumetric indices were used to estimate repeatability of customized grids and compare their performance to standard grids.RESULTS. Test-retest measures of standard-grid MS yielded higher coefficients of variation (CV) in CHM subjects compared with controls (0.09 vs. 0.02). Volumetric indices from customized grids improved repeatability by driving CV values to 0.05 and close to 0.02 for region-of-interest (ROI) analysis. Variability of PWS was significantly higher in CHM, especially at the border of degeneration (10.68 vs. 4.74 dB at the central retina, P < 0.001).CONCLUSIONS. Microperimetry testing in CHM shows high test-retest variation at the border of degeneration, which influences repeatability of MS measures. Volumetric measures from customized grids can improve reliability of both global and regional sensitivity assessment. Nevertheless, inherent test-retest variation of individual points needs to be taken into account when assessing potential functional decline and/or disease progression.