The usefulness of in vitro models to predict the bioavailability of iron and zinc:: A consensus statement from the HarvestPlus expert consultation
The usefulness of in vitro models to predict the bioavailability of iron and zinc:: A consensus statement from the HarvestPlus expert consultation
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DOI：
10.1024/0300-9831.75.6.371
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发表时间：
2005-11-01
影响因子：
2.3
通讯作者：
Trinidad, TP
中科院分区：
文献类型：
--
作者：
Fairweather-Tait, S;Lynch, S;Trinidad, TP
膳食和宿主相关因素的结合决定铁和锌的吸收，并且已经开发了几种体外方法作为评估生物利用度的初步筛选工具。一个专家委员会审查了证据的有效性，并达成了共识。透析性（有或没有模拟消化）提供了一些有用的信息，但不能预测正确的反应大小，有时可能预测错误的反应方向。Caco-2细胞系统（有和没有模拟消化）已经开发出铁的可用性，但不同效果的大小并不总是与人类志愿者获得的结果一致，锌的数据太有限，无法得出关于该方法有效性的结论。不同实验室的Caco-2方法差异很大，需要经验丰富的技术人员和高质量的细胞培养设施才能获得可重复的结果。算法可以提供半定量信息，使饮食能够被分类为高、中等或低生物利用度。虽然体外方法可用于产生想法和发展假设，但它们不能单独用于下列重要决定：食品强化政策，植物育种计划的品种选择，或食品工业的新产品开发。这样的决定最终需要人体研究。
A combination of dietary and host-related factors determines iron and zinc absorption, and several in vitro methods have been developed as preliminary screening tools for assessing bioavailability. An expert committee has reviewed evidence for their usefulness and reached a consensus. Dialyzability (with and without simulated digestion) gives some useful information but cannot predict the correct magnitude of response and may sometimes predict the wrong direction of response. Caco-2 cell systems (with and without simulated digestion) have been developed for iron availability, but the magnitude of different effects does not always agree with results obtained in human volunteers, and the data for zinc are too limited to draw conclusions about the validity of the method. Caco-2 methodologies vary significantly between laboratories and require experienced technicians and good quality cell culture facilities to obtain reproducible results. Algorithms can provide semi-quantitative information enabling diets to be classified as high, moderate, or low bioavailability. While in vitro methods can be used to generate ideas and develop hypotheses, they cannot be used alone for important decisions concerning; food fortification policy, selection of varieties for plant breeding programs, or for new product development in the food industry. Ultimately human studies are required for such determinations.