Whole-Genome Single-Nucleotide-Polymorphism Analysis for Discrimination of Clostridium botulinum Group I Strains
Whole-Genome Single-Nucleotide-Polymorphism Analysis for Discrimination of Clostridium botulinum Group I Strains
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DOI：
10.1128/aem.03934-13
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发表时间：
2014-04-01
影响因子：
4.4
通讯作者：
Sharma, Shashi K.
中科院分区：
文献类型：
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作者：
Gonzalez-Escalona, Narjol;Timme, Ruth;Sharma, Shashi K.
肉毒梭菌是一种遗传多样的革兰氏阳性细菌，可产生极强的神经毒素（肉毒神经毒素A至G [BoNT/A-G]）。三种仅含有BoNT/A1核苷酸序列的菌株的完整基因组序列是公开可得的。虽然这些菌株含有与血凝素基因相关的毒素簇（HA（+）OrfX（-）），但对毒素基因簇中缺乏血凝素基因的A1亚型菌株（称为HA（-）OrfX（+））的基因组知之甚少。我们对三个产BoNT/A1的C.肉毒杆菌菌株：两个菌株具有HA（+）OrfX（-）簇（69 A和32 A），一个菌株具有HA（-）OrfX（+）簇（CDC 297）。这些菌株的全基因组单核苷酸多态性（SNP）分析沿着其他公开可用的C。肉毒杆菌I型菌株揭示了五种不同的谱系。菌株69 A和32A与C. A1型肉毒杆菌属Hall群，CDC 297株与C.肉毒杆菌Ba 4型菌株657。本研究报告了利用全基因组SNP序列分析来区分C。肉毒杆菌I群菌株，并证明了这种分析在快速区分C.携带相同毒素基因亚型的肉毒杆菌菌株。这一分析进一步支持了先前的工作，表明菌株CDC 297和657可能从共同的祖先进化而来，并在不同的基因组位置独立获得单独的BoNT/A1毒素基因簇。
Clostridium botulinum is a genetically diverse Gram-positive bacterium producing extremely potent neurotoxins (botulinum neurotoxins A through G [BoNT/A-G]). The complete genome sequences of three strains harboring only the BoNT/A1 nucleotide sequence are publicly available. Although these strains contain a toxin cluster (HA(+) OrfX(-)) associated with hemagglutinin genes, little is known about the genomes of subtype A1 strains (termed HA(-) OrfX(+)) that lack hemagglutinin genes in the toxin gene cluster. We sequenced the genomes of three BoNT/A1-producing C. botulinum strains: two strains with the HA(+) OrfX(-) cluster (69A and 32A) and one strain with the HA(-) OrfX(+) cluster (CDC297). Whole-genome phylogenic single-nucleotide-polymorphism (SNP) analysis of these strains along with other publicly available C. botulinum group I strains revealed five distinct lineages. Strains 69A and 32A clustered with the C. botulinum type A1 Hall group, and strain CDC297 clustered with the C. botulinum type Ba4 strain 657. This study reports the use of whole-genome SNP sequence analysis for discrimination of C. botulinum group I strains and demonstrates the utility of this analysis in quickly differentiating C. botulinum strains harboring identical toxin gene subtypes. This analysis further supports previous work showing that strains CDC297 and 657 likely evolved from a common ancestor and independently acquired separate BoNT/A1 toxin gene clusters at distinct genomic locations.