Distalization of the Drosophila leg by graded EGF-receptor activity
Distalization of the Drosophila leg by graded EGF-receptor activity
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DOI：
10.1038/nature00971
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发表时间：
2002-08-15
期刊：
影响因子：
64.8
通讯作者：
Campbell, G
中科院分区：
文献类型：
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作者：
Campbell, G
节肢动物和高等脊椎动物都有附肢，但它们在形态上是不同的，并且沿着它们的proximodistal轴（基部到尖端）调节图案的分子机制被认为是完全不同的。在果蝇中，沿着该轴的基因表达沿着被认为主要由转化生长因子-β（TGF-β）和Wnt信号传导的组合控制，所述Wnt信号传导分别来自背条纹和腹条纹中的配体源十肢麻痹（Dpp）和无翼（Wg）（1-3）。然而，在脊椎动物中，近端模式由来自肢芽尖端的配体来源成纤维细胞生长因子（FGF）的受体酪氨酸激酶（RTK）活性调节（4）。在这里，我修改我们的理解，在苍蝇的肢体发育，并表明，远侧区域实际上是由远端到近端梯度的RTK活动，建立了一个来源的表皮生长因子（EGF）相关的配体在假定的尖端图案。脊椎动物和苍蝇的近中端模式之间的这种相似性支持了先前关于动物的附属物/体壁生长之间的进化关系的建议（5，6）。
Arthropods and higher vertebrates both possess appendages, but these are morphologically distinct and the molecular mechanisms regulating patterning along their proximodistal axis (base to tip) are thought to be quite different. In Drosophila, gene expression along this axis is thought to be controlled primarily by a combination of transforming growth factor-beta (TGF-beta) and Wnt signalling from sources of ligands, Decapentaplegic (Dpp) and Wingless (Wg), in dorsal and ventral stripes, respectively(1-3). In vertebrates, however, proximodistal patterning is regulated by receptor tyrosine kinase (RTK) activity from a source of ligands, fibroblast growth factors (FGFs), at the tip of the limb bud(4). Here I revise our understanding of limb development in flies and show that the distal region is actually patterned by a distal-to-proximal gradient of RTK activity, established by a source of epidermal growth factor (EGF)-related ligands at the presumptive tip. This similarity between proximodistal patterning in vertebrates and flies supports previous suggestions(5,6) of an evolutionary relationship between appendages/body-wall outgrowths in animals.